ArticleThe Journal of cell biology2008
Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rate.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 87 citations in OpenAlex.
- Integrated multi-omics characterization across clinically relevant subgroups of long COVID.National science review · 2025Article
- Unveiling the Movement of RanBP1 During the Cell Cycle and Its Interaction with a Cyclin-Dependent Kinase (CDK) in Plants.International journal of molecular sciences · 2024Article
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Augmin is a Ran-regulated spindle assembly factor.The Journal of biological chemistry · 2023Article
- The large cytoplasmic volume of oocyte.The Journal of reproduction and development · 2023Review
- CDK11 negatively regulates Wnt/β-catenin signaling in the endosomal compartment by affecting microtubule stability.Cancer biology & medicine · 2020Article
- CDK11The Journal of biological chemistry · 2019Article
- Cell cycle regulation and hematologic malignancies.Blood science (Baltimore, Md.) · 2019Review
- CDK11 Loss Induces Cell Cycle Dysfunction and Death of BRAF and NRAS Melanoma Cells.Pharmaceuticals (Basel, Switzerland) · 2019Article
- CDK-11-Cyclin L is required for gametogenesis and fertility in C. elegans.Developmental biology · 2018Article
- Review
- Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.The Journal of cell biology · 2018Article
- Identification and characterization of the BmCyclin L1-BmCDK11A/B complex in relation to cell cycle regulation.Cell cycle (Georgetown, Tex.) · 2017Article
- Inhibitors of cyclin-dependent kinases as cancer therapeutics.Pharmacology & therapeutics · 2017Review
- Metaphase Spindle Assembly.Biology · 2017Review
- The emerging roles and therapeutic potential of cyclin-dependent kinase 11 (CDK11) in human cancer.Oncotarget · 2016Review
- The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle.Nature communications · 2015Article
- Article
- Nuclear transport factors: global regulation of mitosis.Current opinion in cell biology · 2015Review
- The biochemistry of mitosis.Cold Spring Harbor perspectives in biology · 2015Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of Ran-guanosine triphosphate (GTP) around chromosomes induces local nucleation and plus end stabilization of microtubules (MTs). The nuclear protein TPX2 is required for RanGTP-dependent MT nucleation. To find the MT stabilizer, we affinity purify nuclear localization signal (NLS)-containing proteins from Xenopus laevis egg extracts. This NLS protein fraction contains the MT stabilization activity. After further purification, we used mass spectrometry to identify proteins in active fractions, including cyclin-dependent kinase 11 (Cdk11). Cdk11 localizes on spindle poles and MTs in Xenopus culture cells and egg extracts. Recombinant Cdk11 demonstrates RanGTP-dependent MT stabilization activity, whereas a kinase-dead mutant does not. Inactivation of Cdk11 in egg extracts blocks RanGTP-dependent MT stabilization and dramatically decreases the spindle assembly rate. Simultaneous depletion of TPX2 completely inhibits centrosome-dependent spindle assembly. Our results indicate that Cdk11 is responsible for RanGTP-dependent MT stabilization around chromosomes and that this local stabilization is essential for normal rates of spindle assembly and spindle function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.