ArticleInfection and immunity2008
Role of RppA in the regulation of polymyxin b susceptibility, swarming, and virulence factor expression in Proteus mirabilis.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- The PhoPQ two-component regulatory system as an important regulator of bacterial stress response.Molecular biology reports · 2025Review
- Prevalence of mutations associated with tolerance to chlorhexidine and other cationic biocides amongMicrobiology (Reading, England) · 2025Article
- In silico investigation and expression analysis of two-component regulatory systems in Proteus mirabilis.Molecular biology reports · 2025Article
- Unveiling the hidden arsenal: new insights intoFrontiers in cellular and infection microbiology · 2024Review
- In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections.Pathogens (Basel, Switzerland) · 2023Review
- Lipopolysaccharide structure modulates cationic biocide susceptibility and crystalline biofilm formation inFrontiers in microbiology · 2023Article
- Silicone Foley catheters impregnated with microbial indole derivatives inhibit crystalline biofilm formation byFrontiers in cellular and infection microbiology · 2022Article
- Into the understanding the multicellular lifestyle ofFrontiers in cellular and infection microbiology · 2022Review
- De-repression of theAntimicrobial agents and chemotherapy · 2019Article
- Molecular mechanisms related to colistin resistance in Enterobacteriaceae.Infection and drug resistance · 2019Article
- MrpJ Directly Regulates Proteus mirabilis Virulence Factors, Including Fimbriae and Type VI Secretion, during Urinary Tract Infection.Infection and immunity · 2018Article
- Swarmer Cell Development of the Bacterium Proteus mirabilis Requires the Conserved Enterobacterial Common Antigen Biosynthesis GeneJournal of bacteriology · 2018Article
- The Rcs regulon in Proteus mirabilis: implications for motility, biofilm formation, and virulence.Current genetics · 2016Article
- Polymyxin: Alternative Mechanisms of Action and Resistance.Cold Spring Harbor perspectives in medicine · 2016Review
- Stenotrophomonas maltophilia PhoP, a Two-Component Response Regulator, Involved in Antimicrobial Susceptibilities.PloS one · 2016Article
- Proteus mirabilis and Urinary Tract Infections.Microbiology spectrum · 2015Review
- New aspects of RpoE in uropathogenic Proteus mirabilis.Infection and immunity · 2015Article
- Serratia marcescens arn, a PhoP-regulated locus necessary for polymyxin B resistance.Antimicrobial agents and chemotherapy · 2014Article
- Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria.Frontiers in microbiology · 2014Review
- Identification of novel factors involved in modulating motility of Salmonella enterica serotype typhimurium.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteus mirabilis, a human pathogen that frequently causes urinary tract infections, is intrinsically highly resistant to cationic antimicrobial peptides, such as polymyxin B (PB). To explore the mechanisms underlying P. mirabilis resistance to PB, a mutant which displayed increased (> 160-fold) sensitivity to PB was identified by transposon mutagenesis. This mutant was found to have Tn5 inserted into a novel gene, rppA. Sequence analysis indicated that rppA may encode a response regulator of the two-component system and is located upstream of the rppB gene, which may encode a membrane sensor kinase. An rppA knockout mutant of P. mirabilis had an altered lipopolysaccharide (LPS) profile. The LPS purified from the rppA knockout mutant could bind more PB than the LPS purified from the wild type. These properties of the rppA knockout mutant may contribute to its PB-sensitive phenotype. The rppA knockout mutant exhibited greater swarming motility and cytotoxic activity and expressed higher levels of flagellin and hemolysin than the wild type, suggesting that RppA negatively regulates swarming, hemolysin expression, and cytotoxic activity in P. mirabilis. PB could modulate LPS synthesis and modification, swarming, hemolysin expression, and cytotoxic activity in P. mirabilis through an RppA-dependent pathway, suggesting that PB could serve as a signal to regulate RppA activity. Finally, we demonstrated that the expression of rppA was up-regulated by a low concentration of PB and down-regulated by a high concentration of Mg2+. Together, these data highlight the essential role of RppA in regulating PB susceptibility and virulence functions in P. mirabilis.
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