ArticleAnnals of botany2008
Agrobacterium rhizogenes-transformed roots of coffee (Coffea arabica): conditions for long-term proliferation, and morphological and molecular characterization.
Article in Annals of botany, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Solanum villosum Mill. an underutilized plant: establishment of hairy root culture and enhanced production of solasodine after elicitor treatment.Protoplasma · 2025Article
- Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis.Plants (Basel, Switzerland) · 2025Review
- Using machine learning to link the influence of transferredFrontiers in plant science · 2022Article
- Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity.Molecular biotechnology · 2019Article
- Coffee Somatic Embryogenesis: How Did Research, Experience Gained and Innovations Promote the Commercial Propagation of Elite Clones From the Two Cultivated Species?Frontiers in plant science · 2018Review
- Article
- A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.Plant cell reports · 2015Article
- Composite potato plants with transgenic roots on non-transgenic shoots: a model system for studying gene silencing in roots.Plant cell reports · 2014Article
- Efficiency of different Agrobacterium rhizogenes strains on hairy roots induction in Solanum mammosum.World journal of microbiology & biotechnology · 2013Article
- Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.Journal of experimental botany · 2012Article
- Kirenol production in hairy root culture of Siegesbeckea orientalis and its antimicrobial activity.Pharmacognosy magazine · 2012Article
- Function of the aux and rol genes of the Ri plasmid in plant cell division in vitro.Plant signaling & behavior · 2009Article
- Phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance.Plant cell reports · 2009Article
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8 authors.
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Abstract
BACKGROUND AND
aimsThe aims of this study were to set up proliferation conditions for hairy roots of Coffea arabica regenerated after transformation by Agrobacterium rhizogenes strain A4-RS, and to carry out the morphological and molecular characterization of hairy root clones maintained over the long term.
methodsAuxin supply, light conditions and sucrose concentration were modified with the aim of establishing efficient root proliferation conditions. The morphological variability among 62 established hairy root clones was phenotyped by scanning the roots and analysing the images using 'whinRHIZO' software procedures. PCR analysis of integration in transformed root cells of rol and aux oncogenes from the T-DNA of the Ri plasmid was used to study the molecular variability among clones. KEY
resultsAuxin supply was necessary to obtain and stimulate growth and branching, and IBA applied at 0.5 microm was the most efficient auxin. Significant differences were shown among the 62 clones for total root length and for the percentage of fine roots. These variables were stable across subcultures and could hence be used for efficient characterization of hairy root clones. The majority of hairy root clones (86 %) exhibited non-significant phenotype differences with non-transformed roots. Eight clones were significantly different from the non-transformed controls in that they possessed a low proportion of fine roots. Two other hairy root clones grew significantly faster than the other clones. The PCR analysis revealed a low variability in the integration of rol and aux oncogenes in transformed root cells. The T(R)-DNA was never integrated as aux1 and aux2 genes were not found, although rolB and rolC genes from the T(L)-DNA were always present.
conclusionsThe discovery of low morphological variability among coffee hairy roots together with the identification of morphological variables allowing easy identification of phenotypically altered clones represent two important results. They make hairy roots a possible, and efficient, tool for functional-genomic studies of coffee root genes.
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