ArticleBiochimica et biophysica acta2008
Focus on RNA isolation: obtaining RNA for microRNA (miRNA) expression profiling analyses of neural tissue.
Article in Biochimica et biophysica acta, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- The Role of MicroRNAs in the Pathogenesis and as Biomarkers for Pediatric Epilepsy: A Systematic Review.Molecular diagnosis & therapy · 2025Pooled it
- Nicorandil treatment improves survival and spatial learning in aged granulin knockout mice.Brain pathology (Zurich, Switzerland) · 2025Article
- Morphine-mediated release of astrocyte-derived extracellular vesicle miR-23a induces loss of pericyte coverage at the blood-brain barrier: Implications for neuroinflammation.Frontiers in cell and developmental biology · 2022Article
- Temporal changes in inflammatory mitochondria-enriched microRNAs following traumatic brain injury and effects of miR-146a nanoparticle delivery.Neural regeneration research · 2021Article
- Bias in RNA-seq Library Preparation: Current Challenges and Solutions.BioMed research international · 2021Review
- The Mitochondria-Associated ER Membranes Are Novel Subcellular Locations Enriched for Inflammatory-Responsive MicroRNAs.Molecular neurobiology · 2020Article
- MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice.International journal of molecular medicine · 2020Article
- microRNAs Biogenesis, Functions and Role in Tumor Angiogenesis.Frontiers in oncology · 2020Review
- Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene.Journal of neuropathology and experimental neurology · 2020Article
- MicroRNA expression patterns in human anterior cingulate and motor cortex: A study of dementia with Lewy bodies cases and controls.Brain research · 2018Article
- Circulating miRNAs as Diagnostic Biomarkers for Parkinson's Disease.Frontiers in neuroscience · 2018Review
- A Customized Quantitative PCR MicroRNA Panel Provides a Technically Robust Context for Studying Neurodegenerative Disease Biomarkers and Indicates a High Correlation Between Cerebrospinal Fluid and Choroid Plexus MicroRNA Expression.Molecular neurobiology · 2017Article
- miR-1 inhibits progression of high-risk papillomavirus-associated human cervical cancer by targeting G6PD.Oncotarget · 2016Article
- High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges.Non-coding RNA · 2016Article
- Mitochondria-associated microRNAs in rat hippocampus following traumatic brain injury.Experimental neurology · 2015Article
- Expression of miR-15/107 family microRNAs in human tissues and cultured rat brain cells.Genomics, proteomics & bioinformatics · 2014Article
- 3' IsomiR species and DNA contamination influence reliable quantification of microRNAs by stem-loop quantitative PCR.PloS one · 2014Article
- Effects of cocaine and withdrawal on the mouse nucleus accumbens transcriptome.Genes, brain, and behavior · 2013Article
- Article
- A study of small RNAs from cerebral neocortex of pathology-verified Alzheimer's disease, dementia with lewy bodies, hippocampal sclerosis, frontotemporal lobar dementia, and non-demented human controls.Journal of Alzheimer's disease : JAD · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are present in all known plant and animal tissues and appear to be somewhat concentrated in the mammalian nervous system. Many different miRNA expression profiling platforms have been described. However, relatively little research has been published to establish the importance of 'upstream' variables in RNA isolation for neural miRNA expression profiling. We tested whether apparent changes in miRNA expression profiles may be associated with tissue processing, RNA isolation techniques, or different cell types in the sample. RNA isolation was performed on a single brain sample using eight different RNA isolation methods, and results were correlated using a conventional miRNA microarray and then cross-referenced to Northern blots. Differing results were seen between samples obtained using different RNA isolation techniques and between microarray and Northern blot results. Another complication of miRNA microarrays is tissue-level heterogeneity of cellular composition. To investigate this phenomenon, miRNA expression profiles were determined and compared between highly-purified primary cerebral cortical cell preparations of rat primary E15-E18 neurons versus rat primary E15-E18 astrocytes. Finally, to assess the importance of dissecting human brain gray matter from subjacent white matter in cerebral cortical studies, miRNA expression profiles were compared between gray matter and immediately contiguous white matter. The results suggest that for microarray studies, cellular composition is important, and dissecting white matter from gray matter improves the specificity of the results. Based on these data, recommendations for miRNA expression profiling in neural tissues, and considerations worthy of further study, are discussed.
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