ArticleJournal of neurochemistry2008
Gephyrin interacts with the glutamate receptor interacting protein 1 isoforms at GABAergic synapses.
Article in Journal of neurochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- LncRNA MSTRG.13,871/miR155-5p/Grip1 network involved in the post-cardiac arrest brain injury.Scientific reports · 2024Article
- WWC2 modulates GABACell reports · 2024Article
- Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters.Cellular and molecular neurobiology · 2022Article
- Gephyrin: a key regulatory protein of inhibitory synapses and beyond.Histochemistry and cell biology · 2018Review
- Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit.The Journal of biological chemistry · 2014Article
- Down-regulation of gephyrin and GABAA receptor subunits during epileptogenesis in the CA1 region of hippocampus.Epilepsia · 2013Article
- The possible role of GABAA receptors and gephyrin in epileptogenesis.Frontiers in cellular neuroscience · 2013Article
- Molecular and functional interaction between protocadherin-γC5 and GABAA receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Ultrastructure of synapses in the mammalian brain.Cold Spring Harbor perspectives in biology · 2012Review
- Article
- Differential regulation of the postsynaptic clustering of γ-aminobutyric acid type A (GABAA) receptors by collybistin isoforms.The Journal of biological chemistry · 2011Article
- Gain-of-function glutamate receptor interacting protein 1 variants alter GluA2 recycling and surface distribution in patients with autism.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain.The Journal of comparative neurology · 2010Article
- GABA(A) Receptor Dynamics and Constructing GABAergic Synapses.Frontiers in molecular neuroscience · 2008Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We have previously shown that the glutamate receptor interacting protein 1 (GRIP1) splice forms GRIP1a/b and GRIP1c4-7 are present at the GABAergic post-synaptic complex. Nevertheless, the role that these GRIP1 protein isoforms play at the GABAergic post-synaptic complex is not known. We are now showing that GRIP1c4-7 and GRIP1a/b interact with gephyrin, the main post-synaptic scaffold protein of GABAergic and glycinergic synapses. Gephyrin coprecipitates with GRIP1c4-7 or GRIP1a/b from rat brain extracts and from extracts of human embryonic kidney 293 cells that have been cotransfected with gephyrin and one of the GRIP1 protein isoforms. Moreover, purified gephyrin binds to purified GRIP1c4-7 or GRIP1a/b, indicating that gephyrin directly interacts with the common region of these GRIP1 proteins, which includes PDZ domains 4-7. An engineered deletion construct of GRIP1a/b (GRIP1a4-7), which both contains the aforementioned common region and binds to gephyrin, targets to the post-synaptic GABAergic complex of transfected cultured hippocampal neurons. In these hippocampal cultures, endogenous gephyrin colocalizes with endogenous GRIP1c4-7 and GRIP1a/b in over 90% of the GABAergic synapses. Double-labeling electron microscopy immunogold reveals that in the rat brain GRIP1c4-7 and GRIP1a/b colocalize with gephyrin at the post-synaptic complex of individual synapses. These results indicate that GRIP1c4-7 and GRIP1a/b colocalize and interact with gephyrin at the GABAergic post-synaptic complex and suggest that this interaction plays a role in GABAergic synaptic function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.