ArticleMolecular and cellular biology1991
trans-dominant mutants of E1A provide genetic evidence that the zinc finger of the trans-activating domain binds a transcription factor.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Article
- The Transcriptional Repressor BS69 is a Conserved Target of the E1A Proteins from Several Human Adenovirus Species.Viruses · 2018Article
- Adenovirus E1A Activation Domain Regulates H3 Acetylation Affecting Varied Steps in Transcription at Different Viral Promoters.Journal of virology · 2018Article
- Review
- The adenoviral oncogene E1A-13S interacts with a specific isoform of the tumor suppressor PML to enhance viral transcription.Journal of virology · 2013Article
- Cellular GCN5 is a novel regulator of human adenovirus E1A-conserved region 3 transactivation.Journal of virology · 2012Article
- Comparison of E1A CR3-dependent transcriptional activation across six different human adenovirus subgroups.Journal of virology · 2010Article
- Requirements for E1A dependent transcription in the yeast Saccharomyces cerevisiae.BMC molecular biology · 2009Article
- Transcriptional control by adenovirus E1A conserved region 3 via p300/CBP.Nucleic acids research · 2009Article
- Identification of a second CtBP binding site in adenovirus type 5 E1A conserved region 3.Journal of virology · 2008Article
- Intrinsic structural disorder in adenovirus E1A: a viral molecular hub linking multiple diverse processes.Journal of virology · 2008Review
- Article
- Roles for APIS and the 20S proteasome in adenovirus E1A-dependent transcription.The EMBO journal · 2006Article
- Recent lessons in gene expression, cell cycle control, and cell biology from adenovirus.Oncogene · 2005Review
- Activation domain-mediator interactions promote transcription preinitiation complex assembly on promoter DNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Molecular anatomy of Tupaia (tree shrew) adenovirus genome; evolution of viral genes and viral phylogeny.Virus genes · 2003Article
- In vivo association of adenovirus large E1A protein with the human mediator complex in adenovirus-infected and -transformed cells.Journal of virology · 2002Article
- Comparative sequence analysis of the largest E1A proteins of human and simian adenoviruses.Journal of virology · 2002Article
- Activation of the Notch-regulated transcription factor CBF1/RBP-Jkappa through the 13SE1A oncoprotein.Genes & development · 2001Article
- The human factors YY1 and LSF repress the human immunodeficiency virus type 1 long terminal repeat via recruitment of histone deacetylase 1.Journal of virology · 2000Article
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2 authors.
Funding
Abstract
The 289R E1A protein of adenovirus stimulates transcription of early viral and certain cellular genes. trans-Activation requires residues 140 to 188, which encompass a zinc finger. Several studies have indicated that trans-activation by E1A is mediated through cellular transcription factors. In particular, the ability of the trans-dominant E1A point mutant hr5 (Ser-185 to Asn) to inhibit wild-type E1A trans-activation was proposed to result from the sequestration of a cellular factor. Using site-directed mutagenesis, we individually replaced every residue within and flanking the trans-activating domain with a conservative amino acid, revealing 16 critical residues. Six of the individual substitutions lying in a contiguous stretch C terminal to the zinc finger (carboxyl region183-188) imparted a trans-dominant phenotype. trans-Dominance was even produced by deletion of the entire carboxyl region183-188. Conversely, an intact finger region147-177 was absolutely required for trans-dominance, since second-site substitution of every critical residue in this region abrogated the trans-dominant phenotype of the hr5 protein. These data indicate that the finger region147-177 bind a limiting cellular transcription factor and that the carboxyl region183-188 provides a separate and essential function. In addition, we show that four negatively charged residues within the trans-activating domain do not comprise a distinct acidic activating region. We present a model in which the trans-activating domain of E1A binds to two different cellular protein targets through the finger and carboxyl regions.
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