ArticleBiochemistry2008
Importance of the maintenance pathway in the regulation of the activity of Escherichia coli ribonucleotide reductase.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- In-Cell Characterization of the Stable Tyrosyl Radical in E. coli Ribonucleotide Reductase Using Advanced EPR Spectroscopy.Angewandte Chemie (International ed. in English) · 2021Article
- Bacterial iron detoxification at the molecular level.The Journal of biological chemistry · 2020Review
- Ribonucleotide Reductases: Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets.Annual review of biochemistry · 2020Review
- Subunit Interaction Dynamics of Class Ia Ribonucleotide Reductases: In Search of a Robust Assay.Biochemistry · 2020Article
- Evidence that a respiratory shield inThe Journal of biological chemistry · 2019Article
- ISCA1 is essential for mitochondrial FeNature communications · 2017Article
- Choosing the right metal: case studies of class I ribonucleotide reductases.The Journal of biological chemistry · 2014Review
- Genetic characterization and role in virulence of the ribonucleotide reductases of Streptococcus sanguinis.The Journal of biological chemistry · 2014Article
- Ribonucleotide reductase metallocofactor: assembly, maintenance and inhibition.Frontiers in biology · 2014Article
- Investigation of in vivo roles of the C-terminal tails of the small subunit (ββ') of Saccharomyces cerevisiae ribonucleotide reductase: contribution to cofactor formation and intersubunit association within the active holoenzyme.The Journal of biological chemistry · 2013Article
- Metallation and mismetallation of iron and manganese proteins in vitro and in vivo: the class I ribonucleotide reductases as a case study.Metallomics : integrated biometal science · 2012Review
- Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.The Journal of biological chemistry · 2011Article
- The alternative aerobic ribonucleotide reductase of Escherichia coli, NrdEF, is a manganese-dependent enzyme that enables cell replication during periods of iron starvation.Molecular microbiology · 2011Article
- Control of metallation and active cofactor assembly in the class Ia and Ib ribonucleotide reductases: diiron or dimanganese?Current opinion in chemical biology · 2011Review
- Escherichia coli class Ib ribonucleotide reductase contains a dimanganese(III)-tyrosyl radical cofactor in vivo.Biochemistry · 2011Article
- Class I ribonucleotide reductases: metallocofactor assembly and repair in vitro and in vivo.Annual review of biochemistry · 2011Review
- A hot oxidant, 3-NO2Y122 radical, unmasks conformational gating in ribonucleotide reductase.Journal of the American Chemical Society · 2010Article
- Site-specific incorporation of 3-nitrotyrosine as a probe of pKa perturbation of redox-active tyrosines in ribonucleotide reductase.Journal of the American Chemical Society · 2010Article
- An active dimanganese(III)-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase.Biochemistry · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Ribonucleotide reductases (RNRs) catalyze the conversion of nucleotides to deoxynucleotides in all organisms. The Escherichia coli class Ia RNR is composed of alpha and beta subunits that form an alpha 2beta 2 active complex. beta contains the diferric tyrosyl radical (Y (*)) cofactor that is essential for the reduction process that occurs on alpha. [Y (*)] in vitro is proportional to RNR activity, and its regulation in vivo potentially represents a mechanism for controlling RNR activity. To examine this thesis, N- and C-terminal StrepII-tagged beta under the control of an l-arabinose promoter were constructed. Using these constructs and with [ l-arabinose] varying from 0 to 0.5 mM in the growth medium, [beta] could be varied from 4 to 3300 microM. [Y (*)] in vivo and on affinity-purified Strep-beta in vitro was determined by EPR spectroscopy and Western analysis. In both cases, there was 0.1-0.3 Y (*) radical per beta. To determine if the substoichiometric Y (*) level was associated with apo beta or diferric beta, titrations of crude cell extracts from these growths were carried out with reduced YfaE, a 2Fe2S ferredoxin involved in cofactor maintenance and assembly. Each titration, followed by addition of O 2 to assemble the cofactor and EPR analysis to quantitate Y (*), revealed that beta is completely loaded with a diferric cluster even when its concentration in vivo is 244 microM. These titrations, furthermore, resulted in 1 Y (*) radical per beta, the highest levels reported. Whole cell Mössbauer analysis on cells induced with 0.5 mM arabinose supports high iron loading in beta. These results suggest that modulation of the level of Y (*) in vivo in E. coli is a mechanism of regulating RNR activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.