ArticleAmerican journal of respiratory cell and molecular biology2008
MARCKS regulation of mucin secretion by airway epithelium in vitro: interaction with chaperones.
Article in American journal of respiratory cell and molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Allergen-induced activation of epithelial P2YThe Journal of allergy and clinical immunology · 2025Article
- Activation of Marck-like Genes and Proteins During Initial Phases of Regeneration in the Amputated Tail and Limb of the LizardJournal of developmental biology · 2025Article
- Phosphorylation-dependent proteome of Marcks in ependyma during aging and behavioral homeostasis in the mouse forebrain.GeroScience · 2022Article
- Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b.PLoS genetics · 2019Article
- MARCKS and Lung Disease.American journal of respiratory cell and molecular biology · 2019Review
- MARCKS and MARCKS-like proteins in development and regeneration.Journal of biomedical science · 2018Review
- Exocytosis of polyubiquitinated proteins in bortezomib-resistant leukemia cells: a role for MARCKS in acquired resistance to proteasome inhibitors.Oncotarget · 2016Article
- Chitin-Induced Airway Epithelial Cell Innate Immune Responses Are Inhibited by Carvacrol/Thymol.PloS one · 2016Article
- Regulated mucin secretion from airway epithelial cells.Frontiers in endocrinology · 2013Review
- MARCKS and HSP70 interactions regulate mucin secretion by human airway epithelial cells in vitro.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Protein kinase C controls vesicular transport and secretion of apolipoprotein E from primary human macrophages.The Journal of biological chemistry · 2013Article
- Calpain and MARCKS protein regulation of airway mucin secretion.Pulmonary pharmacology & therapeutics · 2012Article
- Regulation of mucin secretion and inflammation in asthma: a role for MARCKS protein?Biochimica et biophysica acta · 2011Review
- Mucin granule-associated proteins in human bronchial epithelial cells: the airway goblet cell "granulome".Respiratory research · 2011Article
- Two myristoylated alanine-rich C-kinase substrate (MARCKS) paralogs are required for normal development in zebrafish.Anatomical record (Hoboken, N.J. : 2007) · 2011Article
- Mesenchymal stem cells require MARCKS protein for directed chemotaxis in vitro.American journal of respiratory cell and molecular biology · 2010Article
- MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells.American journal of respiratory cell and molecular biology · 2010Article
- Advances in mucous cell metaplasia: a plug for mucus as a therapeutic focus in chronic airway disease.American journal of respiratory cell and molecular biology · 2010Review
- MARCKS modulates radial progenitor placement, proliferation and organization in the developing cerebral cortex.Development (Cambridge, England) · 2009Article
- Bcl-2 suppresses sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression in cystic fibrosis airways: role in oxidant-mediated cell death.American journal of respiratory and critical care medicine · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have reported previously that myristoylated alanine-rich C kinase substrate (MARCKS) is a key regulatory molecule controlling mucin secretion by airway epithelial cells in vitro and in vivo. The results of those studies supported a mechanism whereby MARCKS, upon phosphorylation by protein kinase C (PKC), translocates from plasma membrane to cytoplasm, where its binding to membranes of intracellular mucin granules is a key component of the secretory pathway. It remains unknown how MARCKS is targeted to and/or preferentially attaches to mucin granule membranes. We hypothesized that the chaperone cysteine string protein (CSP) may play an important role in this process. CSP was shown to associate with membranes of intracellular mucin granules in well-differentiated normal human bronchial epithelial (NHBE) cells in vitro, as determined by ultrastructural immunohistochemistry and Western blotting of isolated granule membranes. CSP in these cells complexed with MARCKS, as shown by co-immunoprecipitation. Given reported associations between CSP and a second chaperone, heat shock protein 70 (HSP70), a role for HSP70 in the MARCKS-dependent secretory mechanism also was investigated. HSP70 appeared to form a trimeric complex with MARCKS and CSP associated with mucin granule membranes within airway epithelial cells. Transfection of the HBE1 human bronchial epithelial cell line with siRNAs targeting sequences of MARCKS, CSP, or HSP70 resulted, in each case, in significant knockdown of expression of these proteins and subsequent attenuation of mucin secretion. The results provide the first evidence that CSP and HSP70, and their interactions with MARCKS, are involved in mucin secretion.
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