ArticleNitric oxide : biology and chemistry2008
Carbon monoxide decreases the level of iNOS protein and active dimer in IL-1beta-stimulated hepatocytes.
Article in Nitric oxide : biology and chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 38 citations in OpenAlex.
- Head-to-Head Comparison of Selected Extra- and Intracellular CO-Releasing Molecules on Their CO-Releasing and Anti-Inflammatory Properties.Chembiochem : a European journal of chemical biology · 2022Article
- The Diverse Roles of Heme Oxygenase-1 in Tumor Progression.Frontiers in immunology · 2021Review
- Remote Ischemic Preconditioning induces Cardioprotective Autophagy and Signals through the IL-6-Dependent JAK-STAT Pathway.International journal of molecular sciences · 2020Article
- P2X7 Receptors Mediate CO-Induced Alterations in Gene Expression in Cultured Cortical Astrocytes-Transcriptomic Study.Molecular neurobiology · 2019Article
- Heme oxygenase/carbon monoxide in the female reproductive system: an overlooked signalling pathway.International journal of biochemistry and molecular biology · 2017Review
- Role of Gasotransmitters in Oxidative Stresses, Neuroinflammation, and Neuronal Repair.BioMed research international · 2017Review
- Biology and Metabolism of Sepsis: Innate Immunity, Bioenergetics, and Autophagy.Surgical infections · 2016Review
- Biological signaling by small inorganic molecules.Coordination chemistry reviews · 2016Article
- Inhaled carbon monoxide protects time-dependently from loss of hypoxic pulmonary vasoconstriction in endotoxemic mice.Respiratory research · 2015Article
- Remote ischemic preconditioning protects against liver ischemia-reperfusion injury via heme oxygenase-1-induced autophagy.PloS one · 2014Article
- Characterization of the proximal ligand in the P420 form of inducible nitric oxide synthase.Journal of the American Chemical Society · 2009Article
- Ketamine-induced hepatoprotection: the role of heme oxygenase-1.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
There is evidence that NO can regulate CO production, however less is known about CO regulation of NO synthesis. Our studies were undertaken to define how CO regulates iNOS in cultured hepatocytes. CO (250ppm) exposure resulted in a significant decrease in iNOS protein, nitrite production, level of active iNOS dimer and cytosolic iNOS activity in cells stimulated with cytokines (IL-1beta) or transfected with the human iNOS gene. However, IL-1beta-stimulated iNOS mRNA expression was unaffected by CO. These effects of CO on iNOS protein levels were inhibited when CO was scavenged using hemoglobin. HO-1 induction with an adenoviral vector carrying HO-1 showed a decrease in total iNOS protein, nitrite production, and iNOS dimer level from cells stimulated by IL-1beta. iNOS protein level was significantly higher in lung endothelial cells isolated from HO-1 knockout mice compared to wild type cultures stimulated with cytokines mixture. CO was found to increase p38 phosphorylation and p38 inhibition using SB203580 increased iNOS protein levels in response to IL-1beta. Interestingly, proteasome inhibitors (MG132 and Lactacystin) and an autophagy inhibitor (3-methyladenine) reversed CO influence iNOS levels. Our results imply that CO exposure decreases NO production by suppressing dimer formation and increasing iNOS degradation through a process involving p38 activation.
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