ArticleProceedings of the National Academy of Sciences of the United States of America1991
Localization of pre-messenger RNA at discrete nuclear sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 130 citations in OpenAlex.
- Article
- An Intermittent Cytochemist.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Single-molecule analysis of endogenous β-actin mRNA trafficking reveals a mechanism for compartmentalized mRNA localization in axons.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cue-Polarized Transport of β-actin mRNA Depends on 3'UTR and Microtubules in Live Growth Cones.Frontiers in cellular neuroscience · 2018Article
- Giant poly(A)-rich RNP aggregates form at terminal regions of avian lampbrush chromosomes.Chromosoma · 2016Article
- RNAs specifically affect gene expression in a length, position and sequence dependent manner.International journal of clinical and experimental pathology · 2014Article
- Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion.Nature communications · 2012Article
- The nucleus introduced.Cold Spring Harbor perspectives in biology · 2011Review
- The nucleolus.Cold Spring Harbor perspectives in biology · 2011Review
- Imaging of single mRNAs in the cytoplasm of living cells.Progress in molecular and subcellular biology · 2004Review
- Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis.The Journal of cell biology · 2002Article
- A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing.Oncogene · 2002Article
- Nuclear relocalization of the pre-mRNA splicing factor PSF during apoptosis involves hyperphosphorylation, masking of antigenic epitopes, and changes in protein interactions.Molecular biology of the cell · 2001Article
- Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.Nucleic acids research · 2001Review
- Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles.Molecular biology of the cell · 2001Article
- The nucleolus and the four ribonucleoproteins of translation.The Journal of cell biology · 2000Review
- Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.Molecular biology of the cell · 2000Article
- Electron tomography reveals posttranscriptional binding of pre-mRNPs to specific fibers in the nucleoplasm.The Journal of cell biology · 2000Article
- Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles.The Journal of cell biology · 1999Article
- Intron-independent association of splicing factors with active genes.The Journal of cell biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the nuclear localization of rhodamine-labeled pre-mRNA after microinjection into nuclei of cultured rat kidney epithelial cells. Intranuclear localization of the injected RNA was followed in the living cells by fluorescence microscopy and digital image processing. Injected human beta-globin pre-mRNA became localized in 30-60 discrete nuclear sites that were coincident with loci defined by monoclonal antibodies against small nuclear ribonucleoproteins (Sm) or another spliceosome component (SC-35) in parallel immunocytochemical studies on the same nuclei. Similar patterns of nuclear localization were observed with a rat proenkephalin pre-mRNA. Nuclear microinjection of an intronlacking beta-globin RNA, a splicing-defective beta-globin mutant pre-mRNA, or an antisense beta-globin pre-mRNA did not result in localization at discrete sites. These results indicate that pre-mRNA binds preferentially to nuclear Sm and SC-35 antibody-reactive sites in vivo and that the binding requires intron sequences.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.