ArticleApplied and environmental microbiology2008
Optical tweezers cause physiological damage to Escherichia coli and Listeria bacteria.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 171 citations in OpenAlex.
- Microfluidic chips in female reproduction: a systematic review of status, advances, and challenges.Theranostics · 2024Pooled it
- Selective photoacoustic handling of microparticles via annular laser beams.Microsystems & nanoengineering · 2026Article
- Flagellar location determines the stability of bacterial surface entrapment.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bacteria Optimize Tumble Bias to Strategically Navigate Surface Constraints.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Streaming-based Tweezers for Routing, Engineering, and Manipulation of multiparticles: STREAM.Microsystems & nanoengineering · 2025Article
- The bacterial flagellum as an object for optical trapping.Biophysical reviews · 2024Review
- Programmable Multimodal Optothermal Manipulation of Synthetic Particles and Biological Cells.ACS nano · 2022Article
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- In vivo acoustic manipulation of microparticles in zebrafish embryos.Science advances · 2022Article
- Unraveling the binding microprocess of individualJournal of oral microbiology · 2022Article
- Potential of the acoustic micromanipulation technologies for biomedical research.Biomicrofluidics · 2021Article
- Opto-refrigerative tweezers.Science advances · 2021Article
- High-Order Fiber Mode Beam Parameter Optimization for Transport and Rotation of Single Cells.Micromachines · 2021Article
- Atomistic modeling and rational design of optothermal tweezers for targeted applications.Nano research · 2021Article
- The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor.Frontiers in microbiology · 2021Review
- Article
- Plasmonic probing of the adhesion strength of single microbial cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Single-Cell Elasticity Measurement with an Optically Actuated Microrobot.Micromachines · 2020Article
- Multiview microscopy of single cells through microstructure-based indirect optical manipulation.Biomedical optics express · 2020Article
- Manipulating rod-shaped bacteria with optical tweezers.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the degree of physiological damage to bacterial cells caused by optical trapping using a 1,064-nm laser. The physiological condition of the cells was determined by their ability to maintain a pH gradient across the cell wall; healthy cells are able to maintain a pH gradient over the cell wall, whereas compromised cells are less efficient, thus giving rise to a diminished pH gradient. The pH gradient was measured by fluorescence ratio imaging microscopy by incorporating a pH-sensitive fluorescent probe, green fluorescent protein or 5(6)-carboxyfluorescein diacetate succinimidyl ester, inside the bacterial cells. We used the gram-negative species Escherichia coli and three gram-positive species, Listeria monocytogenes, Listeria innocua, and Bacillus subtilis. All cells exhibited some degree of physiological damage, but optically trapped E. coli and L. innocua cells and a subpopulation of L. monocytogenes cells, all grown with shaking, showed only a small decrease in pH gradient across the cell wall when trapped by 6 mW of laser power for 60 min. However, another subpopulation of Listeria monocytogenes cells exhibited signs of physiological damage even while trapped at 6 mW, as did B. subtilis cells. Increasing the laser power to 18 mW caused the pH gradient of both Listeria and E. coli cells to decrease within minutes. Moreover, both species of Listeria exhibited more-pronounced physiological damage when grown without shaking than was seen in cells grown with shaking, and the degree of damage is therefore also dependent on the growth conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.