ArticleApplied and environmental microbiology2008
Targeted mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through natural transformation of PCR fragments.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- DNA duplication in Burkholderia thailandensis induces biofilm formation by activating a two-component regulatory system.PLoS genetics · 2025Article
- DNA duplication-mediated activation of a two-component regulatory system serves as a bet-hedging strategy forbioRxiv : the preprint server for biology · 2024Article
- Defluorination of PFAS by Acidimicrobium sp. strain A6 and potential applications for remediation.Methods in enzymology · 2024Article
- NosP Detection of Heme ModulatesBiochemistry · 2023Article
- Article
- Piperacillin triggers virulence factor biosynthesis via the oxidative stress response inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Burkholderia thailandensis Methylated Hydroxyalkylquinolines: Biosynthesis and Antimicrobial Activity in Cocultures.Applied and environmental microbiology · 2020Article
- Reporter-Guided Transposon Mutant Selection for Activation of Silent Gene Clusters in Burkholderia thailandensis.Chembiochem : a European journal of chemical biology · 2020Article
- Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.Microbial biotechnology · 2020Article
- Article
- Efflux Pumps of Burkholderia thailandensis Control the Permeability Barrier of the Outer Membrane.Antimicrobial agents and chemotherapy · 2019Article
- A DedA Family Membrane Protein Is Required forFrontiers in microbiology · 2019Article
- CDI/CDS system-encoding genes of Burkholderia thailandensis are located in a mobile genetic element that defines a new class of transposon.PLoS genetics · 2019Article
- Thiaminase I Provides a Growth Advantage by Salvaging Precursors from Environmental Thiamine and Its Analogs in Burkholderia thailandensis.Applied and environmental microbiology · 2018Article
- The heritable natural competency trait of Burkholderia pseudomallei in other Burkholderia species through comE and crp.Scientific reports · 2018Article
- Determinants of Extreme β-Lactam Tolerance in the Burkholderia pseudomallei Complex.Antimicrobial agents and chemotherapy · 2018Article
- Genome-scale analysis of the genes that contribute to Burkholderia pseudomallei biofilm formation identifies a crucial exopolysaccharide biosynthesis gene cluster.PLoS neglected tropical diseases · 2017Article
- Burkholderia thailandensis: Genetic Manipulation.Current protocols in microbiology · 2017Article
- Article
- Quorum Sensing Influences Burkholderia thailandensis Biofilm Development and Matrix Production.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this property for the rapid generation of marked deletion mutations by using PCR products. The method was employed to create 61 mutant strains. Several selectable elements were employed, including elements carrying loxP and FRT recombinase recognition sites to facilitate resistance marker excision. Chromosomal mutations could also be transferred readily between strains by transformation. The availability of simple procedures for creating defined chromosomal mutations and moving them between strains should facilitate genetic analysis of virulence and other traits of these two Burkholderia species.
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