ArticleApplied and environmental microbiology2008
Targeted inactivation of francisella tularensis genes by group II introns.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Identification of theFrontiers in cellular and infection microbiology · 2025Article
- Identification of the Francisella novicida FTN_0096 as a factor involved in intracellular replication and host response.PloS one · 2025Article
- Arginine 58 is indispensable for proper function of theVirulence · 2022Article
- Identification ofFrontiers in cellular and infection microbiology · 2022Article
- A study of innate immune kinetics reveals a role for a chloride transporter in a virulent Francisella tularensis type B strain.MicrobiologyOpen · 2021Article
- Identification of Membrane-Bound Lytic Murein Transglycosylase A (MltA) as a Growth Factor forFrontiers in cellular and infection microbiology · 2020Article
- A predictedVirulence · 2019Article
- Protective effects of the Francisella tularensis ΔpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques.Scientific reports · 2019Article
- Expression of Francisella pathogenicity island protein intracellular growth locus E (IglE) in mammalian cells is involved in intracellular trafficking, possibly through microtubule organizing center.MicrobiologyOpen · 2019Article
- Soluble lytic transglycosylase SLT of Francisella novicida is involved in intracellular growth and immune suppression.PloS one · 2019Article
- AFrontiers in microbiology · 2019Article
- HU protein is involved in intracellular growth and full virulence of Francisella tularensis.Virulence · 2018Article
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- Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis.PloS one · 2016Article
- Generating Isogenic Deletions (Knockouts) inBio-protocol · 2015Article
- Live attenuated Francisella novicida vaccine protects against Francisella tularensis pulmonary challenge in rats and non-human primates.PLoS pathogens · 2014Article
- Lipidation of the FPI protein IglE contributes to Francisella tularensis ssp. novicida intramacrophage replication and virulence.Pathogens and disease · 2014Article
- Bacterial cellular engineering by genome editing and gene silencing.International journal of molecular sciences · 2014Review
- Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis.Mobile DNA · 2014Article
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Authors and funding
5 authors.
Funding
Abstract
Studies of the molecular mechanisms of pathogenesis of Francisella tularensis, the causative agent of tularemia, have been hampered by a lack of genetic techniques for rapid targeted gene disruption in the most virulent subspecies. Here we describe efficient targeted gene disruption in F. tularensis utilizing mobile group II introns (targetrons) specifically optimized for F. tularensis. Utilizing a targetron targeted to blaB, which encodes ampicillin resistance, we showed that the system works at high efficiency in three different subspecies: F. tularensis subsp. tularensis, F. tularensis subsp. holarctica, and "F. tularensis subsp. novicida." A targetron was also utilized to inactivate F. tularensis subsp. holarctica iglC, a gene required for virulence. The iglC gene is located within the Francisella pathogenicity island (FPI), which has been duplicated in the most virulent subspecies. Importantly, the iglC targetron targeted both copies simultaneously, resulting in a strain mutated in both iglC genes in a single step. This system will help illuminate the contributions of specific genes, and especially those within the FPI, to the pathogenesis of this poorly studied organism.
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