ArticleJournal of bacteriology2008
Expression of the gonococcal global regulatory protein Fur and genes encompassing the Fur and iron regulon during in vitro and in vivo infection in women.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Thiostrepton, a resurging drug inhibiting the stringent response to counteract antibiotic-resistance and expression of virulence determinants inFrontiers in microbiology · 2023Article
- Transcriptional and Translational Responsiveness of the Neisseria gonorrhoeae Type IV Secretion System to Conditions of Host Infections.Infection and immunity · 2021Article
- Article
- The ironclad truth: how in vivo transcriptomics and in vitro mechanistic studies shape our understanding of Neisseria gonorrhoeae gene regulation during mucosal infection.Pathogens and disease · 2017Review
- Iron-regulated small RNA expression as Neisseria gonorrhoeae FA 1090 transitions into stationary phase growth.BMC genomics · 2017Article
- Characterization of the Neisseria gonorrhoeae Iron and Fur Regulatory Network.Journal of bacteriology · 2016Article
- Type I Interferon Induction by Neisseria gonorrhoeae: Dual Requirement of Cyclic GMP-AMP Synthase and Toll-like Receptor 4.Cell reports · 2016Article
- Article
- There is a specific response to pH by isolates of Haemophilus influenzae and this has a direct influence on biofilm formation.BMC microbiology · 2014Article
- Identification of sRNAs expressed by the human pathogen Neisseria gonorrhoeae under disparate growth conditions.Frontiers in microbiology · 2014Article
- Article
- Control of RNA stability by NrrF, an iron-regulated small RNA in Neisseria gonorrhoeae.Journal of bacteriology · 2013Article
- Fur-mediated global regulatory circuits in pathogenic Neisseria species.Journal of bacteriology · 2012Review
- Fur-mediated activation of gene transcription in the human pathogen Neisseria gonorrhoeae.Journal of bacteriology · 2012Article
- Protocol for gene expression profiling using DNA microarrays in Neisseria gonorrhoeae.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Transcriptional profiling of Actinobacillus pleuropneumoniae during the acute phase of a natural infection in pigs.BMC genomics · 2010Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The ferric uptake regulatory protein, Fur, functions as a global regulatory protein of gene transcription in the mucosal pathogen Neisseria gonorrhoeae. We have shown previously that several N. gonorrhoeae Fur-repressed genes are expressed in vivo during mucosal gonococcal infection in men, which suggests that this organism infects in an iron-limited environment and that Fur is expressed under these conditions. In this study we have demonstrated expression of the gonococcal fur gene in vitro, in human cervical epithelial cells, and in specimens from female subjects with uncomplicated gonococcal infection. In vitro studies confirmed that the expression of the gonococcal fur gene was repressed during growth under iron-replete growth conditions but that a basal level of the protein was maintained. Using GFP transcriptional fusions constructed from specific Fur binding sequences within the fur promoter/operator region, we determined that this operator region was functional during N. gonorrhoeae infection of cervical epithelial cells. Furthermore, reverse transcription-PCR analysis, as well as microarray analysis, using a custom Neisseria Fur and iron regulon microarray revealed that several Fur- and iron-regulated genes were expressed during N. gonorrhoeae infection of cervical epithelial cells. Microarray analysis of specimens obtained from female subjects with uncomplicated gonococcal infection corroborated our in vitro findings and point toward a key role of gonococcal Fur- and iron-regulated genes in gonococcal disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.