ArticleJournal of bacteriology2008
Chlamydia pneumoniae GroEL1 protein is cell surface associated and required for infection of HEp-2 cells.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
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- Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein.Journal of cell science · 2024Article
- The secreted host-cell protein clusterin interacts with PmpD and promotesFrontiers in cellular and infection microbiology · 2024Article
- Moonlighting genes harbor antisense ORFs that encode potential membrane proteins.Scientific reports · 2023Article
- Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components.Current microbiology · 2020Review
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- Intracellular proteins moonlighting as bacterial adhesion factors.AIMS microbiology · 2018Review
- Lineage structure of Streptococcus pneumoniae may be driven by immune selection on the groEL heat-shock protein.Scientific reports · 2017Article
- TheFrontiers in cellular and infection microbiology · 2017Article
- The novel chlamydial adhesin CPn0473 mediates the lipid raft-dependent uptake of Chlamydia pneumoniae.Cellular microbiology · 2016Article
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- Essential domains of Anaplasma phagocytophilum invasins utilized to infect mammalian host cells.PLoS pathogens · 2015Article
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- Integration of two ancestral chaperone systems into one: the evolution of eukaryotic molecular chaperones in light of eukaryogenesis.Molecular biology and evolution · 2014Article
- The Chlamydia pneumoniae invasin protein Pmp21 recruits the EGF receptor for host cell entry.PLoS pathogens · 2013Article
- cDNA cloning, overexpression, purification and pharmacologic evaluation for anticancer activity of ribosomal protein L23A gene (RPL23A) from the Giant Panda.International journal of molecular sciences · 2012Article
- Article
- Bacterial virulence in the moonlight: multitasking bacterial moonlighting proteins are virulence determinants in infectious disease.Infection and immunity · 2011Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia pneumoniae is an important obligate intracellular pathogen that replicates within an inclusion in the eukaryotic cell. The initial event of a chlamydial infection is the adherence to and subsequent uptake of the infectious elementary bodies (EBs) by the human cell. These processes require yet-unidentified bacterial and eukaryotic surface proteins. The GroEL1 protein, which exhibits a very strong antigenicity and in vitro can activate various eukaryotic cells, is a potential pathogenicity factor. We localized the protein during the infection process and found it in the inclusion but outside the chlamydial particles. GroEL1 was also localized on the surface of EBs, and the protein could be washed off the EBs. Latex beads coated with recombinantly produced GroEL1 (rGroEL1) bound in a dose-dependent manner to HEp-2 cells. Likewise, GroEL1, when expressed and displayed on the yeast cell surface, mediated adhesion to HEp-2 cells. Interestingly, the homologous GroEL2 and GroEL3 proteins showed no adhesive properties. Incubation of primary umbilical vein endothelial cells with soluble GroEL1 and GroEL1-coated latex beads activated the translocation of the general transcription factor NF-kappaB into the nucleus. Finally, preincubation of HEp-2 cells with rGroEL1 significantly reduced subsequent infection with C. pneumoniae, although adhesion of infectious bacteria to eukaryotic cells was not affected. Taken together, these data support a role for extracellular GroEL1 in the establishment of the chlamydial infection.
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