ArticleBiophysical journal2008
Protein dynamics and stability: the distribution of atomic fluctuations in thermophilic and mesophilic dihydrofolate reductase derived using elastic incoherent neutron scattering.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Small Structural Variations, Large Functional Consequences: Comparative Analysis Reveals Structural Control of Ubiquitylation Site Selection by BRCA1/BARD1.bioRxiv : the preprint server for biology · 2026Article
- Function and Engineering of a Food Enzyme Under Coupled High-Temperature-Pressure Conditions: Insights from Molecular Dynamics Simulation and Experimental Validation.Foods (Basel, Switzerland) · 2025Article
- Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity.Nucleic acids research · 2022Article
- Locking out water at 100°C.Biophysical journal · 2021Article
- Adaptations for Pressure and Temperature in Dihydrofolate Reductases.Microorganisms · 2021Article
- Mesophilic Pyrophosphatase Function at High Temperature: A Molecular Dynamics Simulation Study.Biophysical journal · 2020Article
- Entropic contribution to enhanced thermal stability in the thermostable P450 CYP119.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Determination of Dynamical Heterogeneity from Dynamic Neutron Scattering of Proteins.Biophysical journal · 2018Article
- Conformational preferences underlying reduced activity of a thermophilic ribonuclease H.Journal of molecular biology · 2015Article
- Article
- Thermal adaptation of dihydrofolate reductase from the moderate thermophile Geobacillus stearothermophilus.Biochemistry · 2014Article
- Interface matters: the stiffness route to stability of a thermophilic tetrameric malate dehydrogenase.PloS one · 2014Article
- How conformational flexibility stabilizes the hyperthermophilic elongation factor G-domain.The journal of physical chemistry. B · 2013Article
- Thermostabilization of the β1-adrenergic receptor correlates with increased entropy of the inactive state.The journal of physical chemistry. B · 2013Article
- Bio-protective effects of homologous disaccharides on biological macromolecules.European biophysics journal : EBJ · 2012Article
- Thermophilic proteins: insight and perspective from in silico experiments.Chemical Society reviews · 2012Review
- A temperature-dependent conformational change of NADH oxidase from Thermus thermophilus HB8.Proteins · 2012Article
- The role of large-scale motions in catalysis by dihydrofolate reductase.Journal of the American Chemical Society · 2011Article
- Crowding induces differences in the diffusion of thermophilic and mesophilic proteins: a new look at neutron scattering results.Biophysical journal · 2011Article
- Dynamics of thermodynamically stable, kinetically trapped, and inhibitor-bound states of pepsin.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
The temperature dependence of the dynamics of mesophilic and thermophilic dihydrofolate reductase is examined using elastic incoherent neutron scattering. It is demonstrated that the distribution of atomic displacement amplitudes can be derived from the elastic scattering data by assuming a (Weibull) functional form that resembles distributions seen in molecular dynamics simulations. The thermophilic enzyme has a significantly broader distribution than its mesophilic counterpart. Furthermore, although the rate of increase with temperature of the atomic mean-square displacements extracted from the dynamic structure factor is found to be comparable for both enzymes, the amplitudes are found to be slightly larger for the thermophilic enzyme. Therefore, these results imply that the thermophilic enzyme is the more flexible of the two.
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