ArticleJournal of physiology and biochemistry2007
Physiological and biochemical analysis of L. tredecimguttatus venom collected by electrical stimulation.
Article in Journal of physiology and biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 23 citations in OpenAlex.
- De Novo Transcriptome Analysis of the Venom ofMolecules (Basel, Switzerland) · 2021Article
- Not so Dangerous After All? Venom Composition and Potency of the Pholcid (Daddy Long-Leg) SpiderFrontiers in ecology and evolution · 2019Article
- JZTX-V Targets the Voltage Sensor in Kv4.2 to Inhibit IFrontiers in pharmacology · 2019Article
- Article
- Transcriptome Analysis to Understand the Toxicity of Latrodectus tredecimguttatus Eggs.Toxins · 2016Article
- Review
- Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity.Biological research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The L. tredecimguttatus venom was collected by electrical stimulation and systematically analyzed. Gel electrophoresis and RP-HPLC showed that the venom consisted primarily of proteins with molecular weights above 10 kDa, most of which were high-molecular-mass acidic proteins, with fewer proteins and peptides below 10 kDa. The most abundant proteins in the venom were concentrated at around 100 kDa, which included latrotoxins- the principal toxic components of the venom. Injection of the venom in mice and cockroaches P. americana gave rise to obvious poisoned symptoms, with LD50 values of 0.16 mg/kg and 1.87 microg/g, respectively. Electrophysiological experiments showed that the venom could block the neuromuscular transmission in isolated mouse phrenic nerve-hemidiaphragm and rat vas deferens preparations. The low-molecular-weight fraction (<10 kDa) of the venom had no effect on the transmission. Enzymatic analysis indicated that the venom possess activities of several kinds of hydrolases including hyaluronidase and proteases. These results demonstrated that L. tredecimguttatus venom was basically a large-protein-constituted venom and is one of the most poisonous spider venoms known in the world. The mammalian toxicity of the venom was based on its larger proteins rather than on smaller proteins and peptides, and its hydrolase activities might be involved in the latrodectism. The use of electrical stimulation method to collect the venom has the advantages of avoiding contamination and repeated use of the valuable L. tredecimguttatus venom resources.
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