ArticleThe Journal of biological chemistry2008
A dominant suppressor mutation of the met30 cell cycle defect suggests regulation of the Saccharomyces cerevisiae Met4-Cbf1 transcription complex by Met32.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Glycolysis-dependent sulfur metabolism orchestrates morphological plasticity and virulence in fungi.eLife · 2026Article
- Glutathione reductase plays a role in the metabolism of methylmercury degradation inMicrobiology spectrum · 2025Article
- Evidence for a hydrogen sulfide-sensing E3 ligase in yeast.Genetics · 2024Article
- Overexpression of MET4 Leads to the Upregulation of Stress-Related Genes and Enhanced Sulfite Tolerance inCells · 2022Article
- Methionine Dependence of Cancer.Biomolecules · 2020Review
- Cuminal InhibitsMicroorganisms · 2020Article
- Article
- Functional Analysis of the FZF1 Genes ofFrontiers in microbiology · 2018Article
- Flexible model-based clustering of mixed binary and continuous data: application to genetic regulation and cancer.Nucleic acids research · 2017Article
- YGMD: a repository for yeast cooperative transcription factor sets and their target gene modules.Database : the journal of biological databases and curation · 2017Article
- Article
- Identification of yeast cell cycle regulated genes based on genomic features.BMC systems biology · 2013Article
- Combinatorial control of diverse metabolic and physiological functions by transcriptional regulators of the yeast sulfur assimilation pathway.Molecular biology of the cell · 2012Article
- Characterizing the roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.Molecular biology of the cell · 2012Article
- Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae.Genetics · 2012Article
- A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.Molecular cell · 2010Article
- Dissection of combinatorial control by the Met4 transcriptional complex.Molecular biology of the cell · 2010Article
- Characterizing regulatory path motifs in integrated networks using perturbational data.Genome biology · 2010Article
- The emerging regulatory potential of SCFMet30 -mediated polyubiquitination and proteolysis of the Met4 transcriptional activator.Cell division · 2008Article
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4 authors.
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Abstract
Met30 is the substrate recognition subunit of the essential ubiquitin ligase SCF(Met30). The essential function of Met30 is the inactivation of the Saccharomyces cerevisiae transcription factor Met4, because fully activated Met4 induces a cell cycle arrest. Met4 regulates expression of genes involved in the sulfur assimilation pathway and coordinates the transcriptional program and cell cycle progression in response to cadmium and arsenic stress. Met4 lacks DNA binding activity and requires either Cbf1 or one of the two homologous proteins Met31 and Met32 for promoter association. Accordingly, met4 mutants, cbf1 mutants, and met31 met32 double mutants are methionine auxotroph. We isolated a truncated version of Met32 (Met32(Delta145-192)) as a dominant suppressor of the cell cycle defect of met30 mutants. Expression of Met32(Delta145-192) significantly reduced induction of Met4-regulated genes. Interestingly, both Cbf1- and Met31/32-dependent genes were affected by Met32(Delta145-192). Mechanistically, Met32(Delta145-192) prevented recruitment of Met4 to both Cbf1 and Met31/32-dependent promoters. We further demonstrated that Met32 is part of the Cbf1-Met4 complex bound to Cbf1-recruiting promoter elements and that Met31/32 are required for formation of a stable Met4-Cbf1 transcription complex. These results suggest a regulatory role of Met32 as part of the Cbf1-Met4 complex and provide molecular insight into coordination of cell cycle response and modulation of gene expression programs.
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