ArticleTransgenic research2008
Defence-related gene expression in transgenic lemon plants producing an antimicrobial Trichoderma harzianum endochitinase during fungal infection.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 88 citations in OpenAlex.
- The role of fungal chitinases in the tripartite interactions among insects, plants and entomopathogenic fungi.Pest management science · 2026Review
- Targeted gene expression and associated metabolomic insights into Bacillus subtilis LBS5-mediated suppression of Candidatus liberibacter asiaticus in acid lime (Citrus aurantiifolia (christm.) swingle).World journal of microbiology & biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Strain improvement ofFrontiers in microbiology · 2023Review
- Phytase-ProducingMicroorganisms · 2021Article
- Biotechnological Approaches for Genetic Improvement of Lemon (Plants (Basel, Switzerland) · 2021Review
- Can gene editing reduce postharvest waste and loss of fruit, vegetables, and ornamentals?Horticulture research · 2021Review
- Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus.Plants (Basel, Switzerland) · 2020Review
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
- Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review.3 Biotech · 2017Review
- Trichoderma for climate resilient agriculture.World journal of microbiology & biotechnology · 2017Review
- Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.Plant molecular biology · 2017Article
- Overexpression ofFrontiers in plant science · 2017Article
- Cooperative interaction of antimicrobial peptides with the interrelated immune pathways in plants.Molecular plant pathology · 2016Review
- Expression of the β-1,3-glucanase gene bgn13.1 from Trichoderma harzianum in strawberry increases tolerance to crown rot diseases but interferes with plant growth.Transgenic research · 2015Article
- Conferred resistance to Botrytis cinerea in Lilium by overexpression of the RCH10 chitinase gene.Plant cell reports · 2015Article
- Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterization of the best performing individuals in a field trial.Transgenic research · 2015Article
- Cyclic LIPopeptides from Bacillus subtilis ABS-S14 elicit defense-related gene expression in citrus fruit.PloS one · 2014Article
- Construction of a Streptomyces lydicus A01 transformant with a chit42 gene from Trichoderma harzianum P1 and evaluation of its biocontrol activity against Botrytis cinerea.Journal of microbiology (Seoul, Korea) · 2013Article
- GUS expression in sweet oranges (Citrus sinensis L. Osbeck) driven by three different phloem-specific promoters.Plant cell reports · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents a promising strategy for conferring genetic resistance against a broad range of plant pathogenic fungi. In the present work, two transgenic lemon clones with the chit42 gene from Trichoderma harzianum were tested for resistance to fungal disease and expression level of defence-related genes was evaluated. Different resistance-related processes, such as production of reactive oxygen species (ROS), systemic acquired resistance (SAR) and induced systemic resistance (ISR), were monitored in transgenic and wild type lemon clones inoculated with Botrytis cinerea, the causal agent of grey mould in citrus. Expression of genes that encode gluthatione peroxidase (GPX), a producer of ROS, chitinases, glucanases (SAR), PAL, HPL, and AOS (ISR) was measured by quantitative PCR during the first 24 h after leaf inoculation. Leaves of transgenic lemon plants inoculated with B. cinerea showed significantly less lesion development than wild type leaves. Tissues from detached leaves of different transgenic lemon clones showed a significant correlation between resistance and transgene expression. On the other hand, the over-expression of the transgenic fungal gene enhanced by two-three folds transcript levels of genes associated with enhanced ROS production and ISR establishment, while the expression of native chitinase and glucanase genes involved in SAR was down-regulated.
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