ArticlePlant molecular biology2008
Characterisation of 3' transgene insertion site and derived mRNAs in MON810 YieldGard maize.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- An Integrated Strategy for Analyzing the Complete Complex Integrated Structure of Maize MON810 and Identification of an SNP in External Insertion Sequences.Plants (Basel, Switzerland) · 2024Article
- LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms.Plant biotechnology journal · 2022Article
- Untargeted Proteomics-Based Approach to Investigate Unintended Changes in Genetically Modified Maize for Environmental Risk Assessment Purpose.Frontiers in toxicology · 2021Article
- Full Dynamic Range Quantification using Loop-mediated Amplification (LAMP) by Combining Analysis of Amplification Timing and Variance between Replicates at Low Copy Number.Scientific reports · 2020Article
- Optimised LAMP allows single copy detection of 35Sp and NOSt in transgenic maize using Bioluminescent Assay in Real Time (BART).Scientific reports · 2018Article
- Revisiting Risk Governance of GM Plants: The Need to Consider New and Emerging Gene-Editing Techniques.Frontiers in plant science · 2018Review
- Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties.Environmental sciences Europe · 2016Article
- Chronic Responses of Daphnia magna Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt-Maize Hybrid and its Conventional Near-Isoline.Journal of toxicology and environmental health. Part A · 2015Article
- Mutation scanning in a single and a stacked genetically modified (GM) event by real-time PCR and high resolution melting (HRM) analysis.International journal of molecular sciences · 2014Article
- Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize.Environmental sciences Europe · 2014Article
- The use of massive sequencing to detect differences between immature embryos of MON810 and a comparable non-GM maize variety.PloS one · 2014Article
- The application of GMOs in agriculture and in food production for a better nutrition: two different scientific points of view.Genes & nutrition · 2013Article
- Proteomic analysis of MON810 and comparable non-GM maize varieties grown in agricultural fields.Transgenic research · 2011Article
- Stability of the MON 810 transgene in maize.Plant molecular biology · 2010Article
- Debate on GMOs health risks after statistical findings in regulatory tests.International journal of biological sciences · 2010Article
- Demographic responses of Daphnia magna fed transgenic Bt-maize.Ecotoxicology (London, England) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The construct inserted in YieldGard MON810 maize, produced by Monsanto, contains the CaMV 35S promoter, the hsp70 intron of maize, the cryI(A)b gene for resistance to lepidopterans and the NOS terminator. In a previous work a truncation event at the 3' end of the cryI(A)b gene leading to the complete loss of the NOS terminator was demonstrated. The 3' maize genome junction region was isolated in the same experiment not showing any homology with known sequences. The aim of the experiments here reported was therefore to isolate and characterize a larger portion of the 3' integration junction from genomic DNA of two commercial MON810 maize lines. Specific primers were designed on the 3' integration junction sequence for the amplification of a 476 bp fragment downstream of the sequence previously detected. In silico analysis identified the whole isolated 3' genomic region as a gene putatively coding for the HECT E3 ubiquitin ligase. RT-PCR performed in this region produced cDNA variants of different length. In silico translation of these transcripts identified 2 and 18 putative additional aminoacids in different variants, all derived from the adjacent host genomic sequences, added to the truncated CRY1A protein. These putative recombinant proteins did not show homology with any known protein domains. Our data gave new insights on the genomic organization of MON810 in the YieldGard maize and confirmed the previous suggestion that the integration in the genome of maize caused a complex recombination event without, apparently, interfering with the activity of the partial CRY1A endotoxin and both the vigor and yield of the YieldGard maize.
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