ArticlePlant physiology2008
Proteinase inhibitor from ginkgo seeds is a member of the plant nonspecific lipid transfer protein gene family.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Potato Type I Protease Inhibitor Mediates Host Defence Against Potato Virus X Infection by Interacting With a Viral RNA Silencing Suppressor.Molecular plant pathology · 2025Article
- CTB6 Confers Cold Tolerance at the Booting Stage by Maintaining Tapetum Development in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Roles of non-specific lipid transfer proteins in plant defense: structural and functional perspectives.Frontiers in fungal biology · 2025Review
- The revealing of a novel lipid transfer protein lineage in green algae.BMC plant biology · 2023Article
- Lipid transfer proteins involved in plant-pathogen interactions and their molecular mechanisms.Molecular plant pathology · 2022Review
- Transcriptomic Analysis Reveals the Regulatory Networks and Hub Genes Controlling the Unsaturated Fatty Acid Contents of Developing Seed in Soybean.Frontiers in plant science · 2022Article
- Preparation of a functional beverage with α-glucosidase inhibitory peptides obtained from ginkgo seeds.Journal of food science and technology · 2021Article
- Structural characterization and in vitro lipid binding studies of non-specific lipid transfer protein 1 (nsLTP1) from fennel (Foeniculum vulgare) seeds.Scientific reports · 2020Article
- Article
- Plant lipid transfer proteins: are we finally closing in on the roles of these enigmatic proteins?Journal of lipid research · 2018Review
- Deciphering the Evolution and Development of the Cuticle by Studying Lipid Transfer Proteins in Mosses and Liverworts.Plants (Basel, Switzerland) · 2018Review
- Review
- Genome-wide survey and expression analysis of the putative non-specific lipid transfer proteins in Brassica rapa L.PloS one · 2014Article
- A thermoacidophile-specific protein family, DUF3211, functions as a fatty acid carrier with novel binding mode.Journal of bacteriology · 2013Article
- Study of nsLTPs in Lotus japonicus genome reveal a specific epidermal cell member (LjLTP10) regulated by drought stress in aerial organs with a putative role in cutin formation.Plant molecular biology · 2013Article
- Crystallization and preliminary X-ray crystallographic analysis of dioscorin from Dioscorea japonica.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Identification of genes differentially expressed in cauliflower associated with resistance to Xanthomonas campestris pv. campestris.Molecular biology reports · 2011Article
- Campesin, a thermostable antifungal peptide with highly potent antipathogenic activities.Journal of bioscience and bioengineering · 2009Article
- Arabidopsis LTPG is a glycosylphosphatidylinositol-anchored lipid transfer protein required for export of lipids to the plant surface.The Plant cell · 2009Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 9-kD proteinase inhibitor was isolated from the seeds of ginkgo (Ginkgo biloba) and purified to homogeneity. This protein was revealed to partial-noncompetitively inhibit the aspartic acid proteinase pepsin and the cysteine proteinase papain (inhibition constant = 10(-5)-10(-4) m). The cDNA of the inhibitor was revealed to contain a 357-bp open reading frame encoding a 119-amino acid protein with a potential signal peptide (27 residues), indicating that this protein is synthesized as a preprotein and secreted outside the cells. Semiquantitative reverse transcription-polymerase chain reaction revealed that this gene expresses only in seeds, not in stems, leaves, and roots, suggesting that the protein is involved in seed development and/or germination. The inhibitor showed about 40% sequence homology with type-I nonspecific lipid transfer protein (nsLTP1) from other plant species. Actually, this inhibitor exerted both lipid transfer activity and lipid-binding activity, while the protein did not show any antifungal and antibacterial activities. Furthermore, the site-directed mutagenesis study using a recombinant ginkgo nsLTP1 revealed that proline (Pro)-79 and phenylalanine-80 are important on phospholipid transfer activity and that Pro-79 and isoleucine-82 are essential for the binding activity toward cis-unsaturated fatty acids. On the other hand, the alpha-helical content of P79A and F80A mutants was significantly lower than that of the wild-type protein. It was noteworthy that the papain-inhibitory activity of P79A and F80A mutants was elevated twice as much as that of the wild-type protein. In summary, we concluded that Pro-79 plays a critical role in both the lipid transfer and binding activities of ginkgo nsLTP1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.