ArticleBMC biotechnology2008
Systolic and diastolic ventricular function in zebrafish embryos: influence of norepenephrine, MS-222 and temperature.
Article in BMC biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed.
- An Anesthesia-Free Pharmacological Assay for Zebrafish Heart Failure Models Using a Microfluidic Platform.Biomedicines · 2026Article
- Importance of developmental stage and microenvironment control in Zebrafish larvae cardiovascular studies.PloS one · 2026Article
- Zebrafish as a Versatile Model for Cardiovascular Research: Peering into the Heart of the Matter.Cells · 2025Review
- Optical Elastography for Micropressure Characterization of Zebrafish Embryonic Cardiac Development.Annals of biomedical engineering · 2024Article
- Skeletal Muscle Regeneration in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Optical transparency and label-free vessel imaging of zebrafish larvae in shortwave infrared range as a tool for prolonged studying of cardiovascular system development.Scientific reports · 2022Article
- Semaphorin3f as a cardiomyocyte derived regulator of heart chamber development.Cell communication and signaling : CCS · 2022Article
- Efficacy of Tricaine (MS-222) and Hypothermia as Anesthetic Agents for Blocking Sensorimotor Responses in Larval Zebrafish.Frontiers in veterinary science · 2022Article
- Influence of hypomagnetic field on the heartbeat in zebrafish embryos.Frontiers in physiology · 2022Article
- Rapid Generation of Pigment Free, Immobile Zebrafish Embryos and Larvae in Any Genetic Background Using CRISPR-Cas9 dgRNPs.Zebrafish · 2021Article
- Evaluation of the potential environmental risk from the destination of medicines: an epidemiological and toxicological study.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2021Observational
- PDE10A Inhibition Reduces the Manifestation of Pathology in DMD Zebrafish and Represses the Genetic Modifier PITPNA.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Edible additive effects on zebrafish cardiovascular functionality with hydrodynamic assessment.Scientific reports · 2020Article
- Validating the Paradigm That Biomechanical Forces Regulate Embryonic Cardiovascular Morphogenesis and Are Fundamental in the Etiology of Congenital Heart Disease.Journal of cardiovascular development and disease · 2020Review
- Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart.Nature communications · 2019Article
- Article
- Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development.Current biology : CB · 2019Article
- Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model.Nanomaterials (Basel, Switzerland) · 2019Article
- Influence of blood flow on cardiac development.Progress in biophysics and molecular biology · 2018Review
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundZebrafish are increasingly used to study the influences of gene mutation and manipulation on cardiac development, structure and function. In this study, a video edge detection system was used to characterise, continuously, cardiac ventricle function in 2-5 days old zebrafish embryos embedded in 0.6% agar and examined under light microscopy at room temperature (22 degrees C). Using video edge detection software (IonOptix Inc), the motion of a small region of the cardiac ventricle wall was converted to a continuous chart trace allowing analysis of wall motion amplitude (WMA) and myocardial wall velocity during systole (MWVs) and diastole (MWVd).
resultsCardiac wall motion characteristics changed progressively from day 2 to 5 (WMA, 2-days, 17.6 +/- 4.4 microm vs 5-days, 24.6 +/- 4.7 microm, p < 0.01). MWVd was more rapid than MWVs at all developmental time points. Embryonic hearts were also assessed after increasing concentrations of norepenephrine (NE) and the anaesthetic agent MS222 (tricaine) were added to the bathing water. In response to NE, WMA increased significantly more in 4 day embryos compared with 2 day embryos (change in WMA,13.6 +/- 8.2 microm vs 4.0 +/- 8.8 microm, p = 0.01, respectively) while the decrease in WMA in response to MS222 was similar in both 2 and 4-day embryos. Heart rate, MWVs and MWVd were significantly higher at 28 degrees C compared with 22 degrees C. No differences in cardiac function were observed between AB and Golden strains.
conclusionVideo edge detection appears sufficiently sensitive to detect subtle changes in diastolic and systolic cardiac function during development and changes resulting from pharmacological and environmental interventions. Such measurements could be valuable in assessment of altered cardiac function after genetic manipulation.
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