ArticlePLoS biology2008
VirE2: a unique ssDNA-compacting molecular machine.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Nanopore sensing reveals a preferential pathway for the co-translocational unfolding of a conjugative relaxase-DNA complex.Nucleic acids research · 2023Article
- Primary architecture and energy requirements of Type III and Type IV secretion systems.Frontiers in cellular and infection microbiology · 2023Review
- Article
- The Essential, Ubiquitous Single-Stranded DNA-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2021Article
- OsbZIP81, A Homologue of Arabidopsis VIP1, May Positively Regulate JA Levels by Directly Targetting the Genes in JA Signaling and Metabolism Pathway in Rice.International journal of molecular sciences · 2019Article
- Article
- Translation and folding of single proteins in real time.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Kinetics of DNA uptake during transformation provide evidence for a translocation ratchet mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Visualization of VirE2 protein translocation by the Agrobacterium type IV secretion system into host cells.MicrobiologyOpen · 2014Article
- ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells.PLoS genetics · 2014Article
- The roles of bacterial and host plant factors in Agrobacterium-mediated genetic transformation.The International journal of developmental biology · 2013Review
- Single-molecule views of protein movement on single-stranded DNA.Annual review of biophysics · 2012Review
- Traversing the Cell: Agrobacterium T-DNA's Journey to the Host Genome.Frontiers in plant science · 2012Article
- Characterization of the single stranded DNA binding protein SsbB encoded in the Gonoccocal Genetic Island.PloS one · 2012Article
- New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation.The EMBO journal · 2010Review
- Composite system mediates two-step DNA uptake into Helicobacter pylori.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Agrobacterium tumefaciens and A. rhizogenes use different proteins to transport bacterial DNA into the plant cell nucleus.Microbial biotechnology · 2009Review
- Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence.Cellular microbiology · 2008Review
- IMPa-4, an Arabidopsis importin alpha isoform, is preferentially involved in agrobacterium-mediated plant transformation.The Plant cell · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translocation of single-stranded DNA (ssDNA) across membranes of two cells is a fundamental biological process occurring in both bacterial conjugation and Agrobacterium pathogenesis. Whereas bacterial conjugation spreads antibiotic resistance, Agrobacterium facilitates efficient interkingdom transfer of ssDNA from its cytoplasm to the host plant cell nucleus. These processes rely on the Type IV secretion system (T4SS), an active multiprotein channel spanning the bacterial inner and outer membranes. T4SSs export specific proteins, among them relaxases, which covalently bind to the 5' end of the translocated ssDNA and mediate ssDNA export. In Agrobacterium tumefaciens, another exported protein-VirE2-enhances ssDNA transfer efficiency 2000-fold. VirE2 binds cooperatively to the transferred ssDNA (T-DNA) and forms a compact helical structure, mediating T-DNA import into the host cell nucleus. We demonstrated-using single-molecule techniques-that by cooperatively binding to ssDNA, VirE2 proteins act as a powerful molecular machine. VirE2 actively pulls ssDNA and is capable of working against 50-pN loads without the need for external energy sources. Combining biochemical and cell biology data, we suggest that, in vivo, VirE2 binding to ssDNA allows an efficient import and pulling of ssDNA into the host. These findings provide a new insight into the ssDNA translocation mechanism from the recipient cell perspective. Efficient translocation only relies on the presence of ssDNA binding proteins in the recipient cell that compacts ssDNA upon binding. This facilitated transfer could hence be a more general ssDNA import mechanism also occurring in bacterial conjugation and DNA uptake processes.
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