ArticleProceedings of the National Academy of Sciences of the United States of America2008
The enzyme mechanism of nitrite reductase studied at single-molecule level.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 75 citations in OpenAlex.
- Allosteric Regulation of Photophysics and Binding in Oxazine-macrocycle Complexes at Single-molecule Resolution.Angewandte Chemie (International ed. in English) · 2026Article
- Exploring the Potential of Site-Selective Labeling on a Green Fluorescent Protein Through Lys-His Linchpin-Directed Modification.Sensors (Basel, Switzerland) · 2026Article
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- Single molecule-driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes.Science advances · 2025Article
- Single electron transfer events and dynamical heterogeneity in the small protein azurin fromChemical science · 2019Article
- Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition.Scientific reports · 2019Article
- Nitrite Reductase Activity in Engineered Azurin Variants.Inorganic chemistry · 2016Article
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Enzyme molecules in solitary confinement.Molecules (Basel, Switzerland) · 2014Review
- Bright and fast multicoloured voltage reporters via electrochromic FRET.Nature communications · 2014Article
- Tracking electrons in biological macromolecules: from ensemble to single molecule.Molecules (Basel, Switzerland) · 2014Review
- Differential interaction kinetics of a bipolar structure-specific endonuclease with DNA flaps revealed by single-molecule imaging.PloS one · 2014Article
- Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.Biochemistry · 2013Review
- Probing single biomolecules in solution using the anti-Brownian electrokinetic (ABEL) trap.Accounts of chemical research · 2012Article
- Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Heterogeneous properties of individual molecules of beta-galactosidase from the thermophilic bacteria Geobacillus stearothermophilus.The protein journal · 2010Article
- Demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases.The Journal of biological chemistry · 2009Article
- Ultrasensitive measurements of microbial rhodopsin photocycles using photochromic FRET.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A generic method is described for the fluorescence "readout" of the activity of single redox enzyme molecules based on Förster resonance energy transfer from a fluorescent label to the enzyme cofactor. The method is applied to the study of copper-containing nitrite reductase from Alcaligenes faecalis S-6 immobilized on a glass surface. The parameters extracted from the single-molecule fluorescence time traces can be connected to and agree with the macroscopic ensemble averaged kinetic constants. The rates of the electron transfer from the type 1 to the type 2 center and back during turnover exhibit a distribution related to disorder in the catalytic site. The described approach opens the door to single-molecule mechanistic studies of a wide range of redox enzymes and the precise investigation of their internal workings.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.