ArticleBiochemistry2008
Effects of protein oxidation on the structure and stability of model discoidal high-density lipoproteins.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Healthier Lipid Profiles of Japanese Adults, Especially in Women with Elevated High-Density Lipoprotein Cholesterol (HDL-C), Are Associated with Low HDL-C Peroxide Content.Antioxidants (Basel, Switzerland) · 2024Article
- Protective Effects of Four Natural Antioxidants on Hydroxyl-Radical-Induced Lipid and Protein Oxidation in Yak Meat.Foods (Basel, Switzerland) · 2022Article
- Cross-linking modifications of HDL apoproteins by oxidized phospholipids: structural characterization,The Journal of biological chemistry · 2020Article
- Paradoxical effects of SAA on lipoprotein oxidation suggest a new antioxidant function for SAA.Journal of lipid research · 2016Article
- Optimized negative staining: a high-throughput protocol for examining small and asymmetric protein structure by electron microscopy.Journal of visualized experiments : JoVE · 2014Article
- Amyloidogenic mutations in human apolipoprotein A-I are not necessarily destabilizing - a common mechanism of apolipoprotein A-I misfolding in familial amyloidosis and atherosclerosis.The FEBS journal · 2014Article
- Hot spots in apolipoprotein A-II misfolding and amyloidosis in mice and men.FEBS letters · 2014Article
- Optimized negative-staining electron microscopy for lipoprotein studies.Biochimica et biophysica acta · 2013Review
- Hypochlorite-induced oxidative stress elevates the capability of HDL in promoting breast cancer metastasis.Journal of translational medicine · 2012Article
- High Density Lipoprotein and it's Dysfunction.The open biochemistry journal · 2012Article
- Effects of oxidation on structural stability and remodeling of human very low density lipoprotein.Biochemistry · 2010Article
- An optimized negative-staining protocol of electron microscopy for apoE4 POPC lipoprotein.Journal of lipid research · 2010Article
- In vivo administration of BL-3050: highly stable engineered PON1-HDL complexes.BMC clinical pharmacology · 2009Article
- Maturation of high-density lipoproteins.Journal of the Royal Society, Interface · 2009Article
- HDL: bridging past and present with a look at the future.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
High-density lipoproteins (HDLs) prevent atherosclerosis by removing cholesterol from macrophages and by providing antioxidants for low-density lipoproteins. Oxidation of HDLs affects their functions via the complex mechanisms that involve multiple protein and lipid modifications. To differentiate between the roles of oxidative modifications in HDL proteins and lipids, we analyzed the effects of selective protein oxidation by hypochlorite (HOCl) on the structure, stability, and remodeling of discoidal HDLs reconstituted from human apolipoproteins (A-I, A-II, or C-I) and phosphatidylcholines. Gel electrophoresis and electron microscopy revealed that, at ambient temperatures, protein oxidation in discoidal complexes promotes their remodeling into larger and smaller particles. Thermal denaturation monitored by far-UV circular dichroism and light scattering in melting and kinetic experiments shows that protein oxidation destabilizes discoidal lipoproteins and accelerates protein unfolding, dissociation, and lipoprotein fusion. This is likely due to the reduced affinity of the protein for lipid resulting from oxidation of Met and aromatic residues in the lipid-binding faces of amphipathic alpha-helices and to apolipoprotein cross-linking into dimers and trimers on the particle surface. We conclude that protein oxidation destabilizes HDL disk assembly and accelerates its remodeling and fusion. This result, which is not limited to model discoidal but also extends to plasma spherical HDL, helps explain the complex effects of oxidation on plasma lipoproteins.
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Registered trials
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