ArticleEuropean biophysics journal : EBJ2008
Analysis of membrane-localized binding kinetics with FRAP.
Article in European biophysics journal : EBJ, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Inferences from FRAP data are model dependent: A subdiffusive analysis.Biophysical journal · 2022Article
- Supported Lipid Bilayers and the Study of Two-Dimensional Binding Kinetics.Frontiers in molecular biosciences · 2022Review
- A Highly Accurate Pixel-Based FRAP Model Based on Spectral-Domain Numerical Methods.Biophysical journal · 2019Article
- Frapid: achieving full automation of FRAP for chemical probe validation.Biomedical optics express · 2016Article
- Ultrasensitivity in multisite phosphorylation of membrane-anchored proteins.Biophysical journal · 2011Article
- A quantitative approach to analyze binding diffusion kinetics by confocal FRAP.Biophysical journal · 2010Article
- Analysis of the diffusion of Ras2 in Saccharomyces cerevisiae using fluorescence recovery after photobleaching.Physical biology · 2010Article
- A role for rebinding in rapid and reliable T cell responses to antigen.PLoS computational biology · 2009Article
- Convolution-based one and two component FRAP analysis: theory and application.European biophysics journal : EBJ · 2009Article
- Analysis of serial engagement and peptide-MHC transport in T cell receptor microclusters.Biophysical journal · 2008Article
- Effects of intracellular calcium and actin cytoskeleton on TCR mobility measured by fluorescence recovery.PloS one · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interactions between plasma membrane-associated proteins on interacting cells are critical for many important biological processes. Few experimental techniques, however, can accurately determine the association and the dissociation rates between such interacting pairs when the two molecules diffuse on apposing membranes or lipid bilayers. In this study, we give a theoretical description of how and when fluorescence recovery after photobleaching (FRAP) experiments can be used to quantify these reaction rates. We analyze the effect of binding on FRAP recovery curves with a reaction-diffusion model and systematically identify different regimes in the parameter space of the association and the dissociation constants for which the full model simplifies into equivalent one-parameter models. Based on this analysis, we propose an experimental protocol that may be used to identify the kinetic parameters of binding in the appropriate parameter regime. We present simulated experiments illustrating our protocol and lay down guidelines for parameter estimation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.