ArticleProceedings of the National Academy of Sciences of the United States of America2008
Monitoring RNA transcription in real time by using surface plasmon resonance.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- In-process analytics for IVT mRNA manufacturing: from process understanding to advanced process control.Frontiers in bioengineering and biotechnology · 2026Review
- Dioxin and Related Compound Detection: Perspectives for Optical Monitoring.International journal of molecular sciences · 2019Review
- Polymerase/DNA interactions and enzymatic activity: multi-parameter analysis with electro-switchable biosurfaces.Scientific reports · 2015Article
- Detecting sequence dependent transcriptional pauses from RNA and protein number time series.BMC bioinformatics · 2012Article
- Development of a "modular" scheme to describe the kinetics of transcript elongation by RNA polymerase.Biophysical journal · 2011Article
- Fitting experimental transcription data with a comprehensive template-dependent modular kinetic model.Biophysical journal · 2011Article
- Stochastic sequence-level model of coupled transcription and translation in prokaryotes.BMC bioinformatics · 2011Article
- Effects of transcriptional pausing on gene expression dynamics.PLoS computational biology · 2010Article
- Macromolecular micromovements: how RNA polymerase translocates.Current opinion in structural biology · 2009Review
- Quantum mechanics/molecular mechanics investigation of the mechanism of phosphate transfer in human uridine-cytidine kinase 2.Organic & biomolecular chemistry · 2009Article
- Binding of the unorthodox transcription activator, Crl, to the components of the transcription machinery.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The decision to elongate or terminate the RNA chain at specific DNA template positions during transcription is kinetically regulated, but the methods used to measure the rates of these processes have not been sufficiently quantitative to permit detailed mechanistic analysis of the steps involved. Here, we use surface plasmon resonance (SPR) technology to monitor RNA transcription by Escherichia coli RNA polymerase (RNAP) in solution and in real time. We show that binding of RNAP to immobilized DNA templates to form active initiation or elongation complexes can be resolved and monitored by this method, and that changes during transcription that involve the gain or loss of bound mass, including the release of the sigma factor during the initiation-elongation transition, the synthesis of the RNA transcript, and the release of core RNAP and nascent RNA at intrinsic terminators, can all be observed. The SPR method also permits the discrimination of released termination products from paused and other intermediate complexes at terminators. We have used this approach to show that the rate constant for transcript release at intrinsic terminators tR2 and tR' is approximately 2-3 s(-1) and that the extent of release at these terminators is consistent with known termination efficiencies. Simulation techniques have been used to fit the measured parameters to a simple kinetic model of transcription and the implications of these results for transcriptional regulation are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.