ArticleMolecular imaging and biology
Carboxylated superparamagnetic iron oxide particles label cells intracellularly without transfection agents.
Article in Molecular imaging and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Utilization of nanomaterials in MRI contrast agents and their role in therapy guided by imaging.Frontiers in bioengineering and biotechnology · 2024Review
- Cellular and Molecular Processes Are Differently Influenced in Primary Neural Cells by Slight Changes in the Physicochemical Properties of Multicore Magnetic Nanoparticles.ACS applied materials & interfaces · 2023Article
- Prodigious therapeutic effects of combining mesenchymal stem cells with magnetic nanoparticles.World journal of stem cells · 2022Review
- Magnetic, biocompatible FeCOJournal of nanobiotechnology · 2022Article
- A comprehensive study on 2D, 3D and solid tumor environment to explore a multifunctional biogenic nanoconjugate.Scientific reports · 2021Article
- Superparamagnetic Iron Oxide Nanoparticles: Cytotoxicity, Metabolism, and Cellular Behavior in Biomedicine Applications.International journal of nanomedicine · 2021Review
- A Perspective on Cell Tracking with Magnetic Particle Imaging.Tomography (Ann Arbor, Mich.) · 2020Article
- Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters.Scientific reports · 2020Article
- Bifunctional Labeling of Rabbit Mesenchymal Stem Cells for MR Imaging and Fluorescence Microscopy.Molecular imaging and biology · 2020Article
- Instant labeling of therapeutic cells for multimodality imaging.Theranostics · 2020Article
- Janus Iron Oxides @ Semiconducting Polymer Nanoparticle Tracer for Cell Tracking by Magnetic Particle Imaging.Nano letters · 2018Article
- Novel magnetic multicore nanoparticles designed for MPI and other biomedical applications: From synthesis to first in vivo studies.PloS one · 2018Article
- Two novel direct SPIO labels and in vivo MRI detection of labeled cells after acute myocardial infarct.Acta radiologica open · 2017Article
- Targeted transplantation of iron oxide-labeled, adipose-derived mesenchymal stem cells in promoting meniscus regeneration following a rabbit massive meniscal defect.Experimental and therapeutic medicine · 2016Article
- Controlled Phase and Tunable Magnetism in Ordered Iron Oxide Nanotube Arrays Prepared by Atomic Layer Deposition.Scientific reports · 2016Article
- Quantitative Magnetic Particle Imaging Monitors the Transplantation, Biodistribution, and Clearance of Stem Cells In Vivo.Theranostics · 2016Article
- Nanotechnology-Based Drug Delivery Systems for Melanoma Antitumoral Therapy: A Review.BioMed research international · 2015Review
- New findings about iron oxide nanoparticles and their different effects on murine primary brain cells.International journal of nanomedicine · 2015Article
- Chemical basis of interactions between engineered nanoparticles and biological systems.Chemical reviews · 2014Review
- Imaging the intracellular degradation of biodegradable polymer nanoparticles.Beilstein journal of nanotechnology · 2014Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell labeling by superparamagnetic iron oxide particles (SPIO) has emerged as a potentially powerful tool to monitor trafficking of transplanted cells by magnetic resonance tomography, e.g., in studies for tissue repair. However, intracellular labeling is mostly achieved by transfection agents not approved for clinical use. In this work, the feasibility and efficiency of labeling human mesenchymal stem cells (MSC) and HeLa cells with two commercially available SPIOs (Resovist and Feridex) without transfection agents was evaluated. In both cell types, Resovist without a transfection agent was more efficiently taken up than Feridex. Increasing the concentration of Resovist can yield similar amounts of iron in cells as SPIOs with transfection agents. This offers the opportunity to omit transfection agents from the labeling protocol when Resovist is used. Intracellular localization of the contrast agents is found by light microscopy and confirmed by electron microscopy. Coagulation of the SPIO nanoparticles, which is problematic for the quantification of the intracellular iron content, was observed and analyzed with a fluorescent activated cell sorter. As Resovist consists of a carboxydextran shell in contrast to Feridex which is composed of a dextran shell, we synthesized fluorescent polymeric nanoparticles as model systems with different amounts of carboxyl groups on the surface by the miniemulsion process. A steady increase in uptake of nanoparticles was detected with a higher density of carboxyl groups showing the relevance of charged groups as in the case of Resovist. Aggregation of these polymeric nanoparticles was not found.
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