ArticleEukaryotic cell2008
Cell cycle synchrony in Giardia intestinalis cultures achieved by using nocodazole and aphidicolin.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Yeast-derived glycolipids disrupt Candida biofilm and inhibit expression of genes in cell adhesion.Scientific reports · 2025Article
- Antiparasitic activity of Colombian Amazon palm extracts againstFrontiers in microbiology · 2025Article
- Identification of target genes regulated by encystation-induced transcription factor Myb2 using knockout mutagenesis in Giardia lamblia.Parasites & vectors · 2022Article
- Evidence of Non-microtubule Spindle Forces inFrontiers in molecular biosciences · 2020Article
- Role of gamma-giardin in ventral disc formation of Giardia lamblia.Parasites & vectors · 2019Article
- RNA-sequencing Profiles of Cell Cycle-Related Genes Upregulated during the G2-Phase in Giardia lamblia.The Korean journal of parasitology · 2019Article
- Precocious cleavage furrows simultaneously move and ingress when kinetochore microtubules are depolymerized in Mesostoma ehrenbergii spermatocytes.Protoplasma · 2018Article
- ZFP36L2 is a cell cycle-regulated CCCH protein necessary for DNA lesion-induced S-phase arrest.Biology open · 2018Article
- Mad2, Bub3, and Mps1 regulate chromosome segregation and mitotic synchrony in Giardia intestinalis, a binucleate protist lacking an anaphase-promoting complex.Molecular biology of the cell · 2014Article
- Epidemiology of Giardia duodenalis infection in ruminant livestock and children in the Ismailia province of Egypt: insights by genetic characterization.Parasites & vectors · 2014Article
- Characterization of microtubule-binding and dimerization activity of Giardia lamblia end-binding 1 protein.PloS one · 2014Article
- The Giardia cell cycle progresses independently of the anaphase-promoting complex.Journal of cell science · 2013Article
- Regulation of a Myb transcription factor by cyclin-dependent kinase 2 in Giardia lamblia.The Journal of biological chemistry · 2012Article
- Nuclear targeted silver nanospheres perturb the cancer cell cycle differently than those of nanogold.Bioconjugate chemistry · 2011Article
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- Dual acylation accounts for the localization of {alpha}19-giardin in the ventral flagellum pair of Giardia lamblia.Eukaryotic cell · 2009Article
- Identification of growth inhibiting compounds in a Giardia lamblia high-throughput screen.Molecular and biochemical parasitology · 2008Article
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3 authors.
Funding
Abstract
Giardia intestinalis is a ubiquitous intestinal protozoan parasite and has been proposed to represent the earliest diverging lineage of extant eukaryotes. Despite the importance of Giardia as a model organism, research on Giardia has been hampered by an inability to achieve cell cycle synchrony for in vitro cultures. This report details successful methods for attaining cell cycle synchrony in Giardia cultures. The research presented here demonstrates reversible cell cycle arrest in G(1)/S and G(2)/M with aphidicolin and nocodazole, respectively. Following synchronization, cells were able to recover completely from drug treatment and remained viable and maintained synchronous growth for 6 h. These techniques were used to synchronize Giardia cultures to increase the percentages of mitotic spindles in the cultures. This method of synchronization will enhance our ability to study cell cycle-dependent processes in G. intestinalis.
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