ArticleEukaryotic cell2008
Phenotype of a mechanosensitive channel mutant, mid-1, in a filamentous fungus, Neurospora crassa.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- CaJournal of fungi (Basel, Switzerland) · 2022Article
- It's All in the Genes: The Regulatory Pathways of Sexual Reproduction in Filamentous Ascomycetes.Genes · 2019Review
- Calcium Transport Proteins in Fungi: The Phylogenetic Diversity of Their Relevance for Growth, Virulence, and Stress Resistance.Frontiers in microbiology · 2019Review
- A-to-I RNA editing is developmentally regulated and generally adaptive for sexual reproduction inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Thigmo Responses: The Fungal Sense of Touch.Microbiology spectrum · 2017Review
- Tissue mechanics regulate brain development, homeostasis and disease.Journal of cell science · 2017Review
- Article
- Components of the calcium-calcineurin signaling pathway in fungal cells and their potential as antifungal targets.Eukaryotic cell · 2015Review
- Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance.Journal of microbiology (Seoul, Korea) · 2015Article
- Deletion of homologs of the SREBP pathway results in hyper-production of cellulases in Neurospora crassa and Trichoderma reesei.Biotechnology for biofuels · 2015Article
- Activation of a TRP-like channel and intracellular Ca2+ dynamics during phospholipase-C-mediated cell death.Journal of cell science · 2014Article
- FigA, a putative homolog of low-affinity calcium system member Fig1 in Saccharomyces cerevisiae, is involved in growth and asexual and sexual development in Aspergillus nidulans.Eukaryotic cell · 2014Article
- The involvement of the Mid1/Cch1/Yvc1 calcium channels in Aspergillus fumigatus virulence.PloS one · 2014Article
- Neurospora crassa ncs-1, mid-1 and nca-2 double-mutant phenotypes suggest diverse interaction among three Ca(2+)-regulating gene products.Journal of genetics · 2013Article
- Cch1 and Mid1 are functionally required for vegetative growth under low-calcium conditions in the phytopathogenic ascomycete Botrytis cinerea.Eukaryotic cell · 2013Article
- Role of Fig1, a component of the low-affinity calcium uptake system, in growth and sexual development of filamentous fungi.Eukaryotic cell · 2012Article
- Electrical phenotypes of calcium transport mutant strains of a filamentous fungus, Neurospora crassa.Eukaryotic cell · 2012Article
- Article
- How does a hypha grow? The biophysics of pressurized growth in fungi.Nature reviews. Microbiology · 2011Review
- Mid1, a mechanosensitive calcium ion channel, affects growth, development, and ascospore discharge in the filamentous fungus Gibberella zeae.Eukaryotic cell · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the yeast Saccharomyces cerevisiae, the MID1 (mating-induced death) gene encodes a stretch-activated channel which is required for successful mating; the mutant phenotype is rescued by elevated extracellular calcium. Homologs of the MID1 gene are found in fungi that are morphologically complex compared to yeast, both Basidiomycetes and Ascomycetes. We explored the phenotype of a mid-1 knockout mutant in the filamentous ascomycete Neurospora crassa. The mutant exhibits lower growth vigor than the wild type (which is not rescued by replete calcium) and mates successfully. Thus, the role of the MID-1 protein differs from that of the homologous gene product in yeast. Hyphal cytology, growth on diverse carbon sources, turgor regulation, and circadian rhythms of the mid-1 mutant are all similar to those of the wild type. However, basal turgor is lower than wild type, as is the activity of the plasma membrane H(+)-ATPase (measured by cyanide [CN(-)]-induced depolarization of the energy-dependent component of the membrane potential). In addition, the mutant is unable to grow at low extracellular Ca(2+) levels or when cytoplasmic Ca(2+) is elevated with the Ca(2+) ionophore A23187. We conclude that the MID-1 protein plays a role in regulation of ion transport via Ca(2+) homeostasis and signaling. In the absence of normal ion transport activity, the mutant exhibits poorer growth.
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