Evidence map›Paper›PMID 18296535›Full record

ArticleApplied and environmental microbiology2008

Species-specific identification of Campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene.

Susumu Kawasaki, Pina M Fratamico, Irene V Wesley, Shinichi Kawamoto

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. International journal of microbiology · 2022
    Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Susumu KawasakiNational Food Research Institute, Food Hygiene Team, 2-1-12 Kannondai, Tsukuba 305-8642, Japan. skawasa@affrc.go.jp
Pina M Fratamico
Irene V Wesley
Shinichi Kawamoto

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and species-specific PCR assays based on the gyrB gene provide valuable tools for rapid and unambiguous identification of the majority of Campylobacter species.

Indexed as

Polymorphism, Restriction Fragment LengthAnimalsBacterial ProteinsCampylobacterCampylobacter InfectionsDNA, BacterialDNA GyraseDNA PrimersHumansMolecular Sequence DataPolymerase Chain ReactionSequence Analysis, DNASpecies SpecificityBacterial ProteinsDNA, BacterialDNA GyraseDNA Primers

Identifiers

PMID18296535
PMCPMC2293133

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.