ArticleJournal of bacteriology2008
Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Accelerated Adaptive Evolution of Gram-Negative Bacteria With a Broad-Host-Range Dual Base Editor.Microbial biotechnology · 2026Article
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- Diversity in Genetic Regulation of Bacterial Fimbriae Assembled by the Chaperone Usher Pathway.International journal of molecular sciences · 2022Review
- FoxR is an AraC-like transcriptional regulator of ferrioxamine uptake in Salmonella enterica.Molecular microbiology · 2022Article
- Combinatorial pathway balancing provides biosynthetic access to 2-fluoro-Chem catalysis · 2021Article
- DbdR, a New Member of the LysR Family of Transcriptional Regulators, Coordinately Controls Four Promoters in the Thauera aromatica AR-1 3,5-Dihydroxybenzoate Anaerobic Degradation Pathway.Applied and environmental microbiology · 2019Article
- Succinate production positively correlates with the affinity of the global transcription factor Cra for its effector FBP inBiotechnology for biofuels · 2016Article
- Transcription factor-based biosensors enlightened by the analyte.Frontiers in microbiology · 2015Review
- Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter.BMC microbiology · 2013Article
- Finely tuned regulation of the aromatic amine degradation pathway in Escherichia coli.Journal of bacteriology · 2013Article
- Mutational analysis of the N-terminal domain of UreR, the positive transcriptional regulator of urease gene expression.Microbiological research · 2012Article
- The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene.BMC systems biology · 2011Article
- Sequential XylS-CTD binding to the Pm promoter induces DNA bending prior to activation.Journal of bacteriology · 2010Article
- Virulence regulation in Citrobacter rodentium: the art of timing.Microbial biotechnology · 2010Review
- Article
- Nitrilase enzymes and their role in plant-microbe interactions.Microbial biotechnology · 2009Review
- Anti-activator ExsD forms a 1:1 complex with ExsA to inhibit transcription of type III secretion operons.The Journal of biological chemistry · 2009Article
- Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
XylS, an AraC family protein, activates transcription from the benzoate degradation pathway Pm promoter in the presence of a substrate effector such as 3-methylbenzoate (3MB). We developed a procedure to obtain XylS-enriched preparations which proved suitable to analyze its activation mechanism. XylS showed specific 3MB-independent binding to its target operator, which became strictly 3MB dependent in a dimerization-defective mutant. We demonstrated that the N-terminal domain of the protein can make linker-independent interactions with the C-terminal domain and inhibit its capacity to bind DNA. Interactions are hampered in the presence of 3MB effector. We propose two independent roles for 3MB in XylS activation: in addition to its known influence favoring protein dimerization, the effector is able to modify XylS conformation to trigger N-terminal domain intramolecular derepression. We also show that activation by XylS involves RNA polymerase recruitment to the Pm promoter as demonstrated by chromatin immunoprecipitation assays. RNA polymerase switching in Pm transcription was reproduced in in vitro transcription assays. All sigma(32)-, sigma(38)-, and sigma(70)-dependent RNA polymerases were able to carry out Pm transcription in a rigorous XylS-dependent manner, as demonstrated by the formation of open complexes only in the presence of the regulator.
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