ArticlePlasmid2008
New pSC101-derivative cloning vectors with elevated copy numbers.
Article in Plasmid, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
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- The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate.The EMBO journal · 2024Article
- Repurposing the mammalian RNA-binding protein Musashi-1 as an allosteric translation repressor in bacteria.eLife · 2024Article
- Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains.Nature communications · 2022Article
- Regulation of l- and d-Aspartate Transport and Metabolism in Acinetobacter baylyi ADP1.Applied and environmental microbiology · 2022Article
- Directed evolution of rRNA improves translation kinetics and recombinant protein yield.Nature communications · 2021Article
- Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters.ACS synthetic biology · 2021Article
- Orthogonal translation enables heterologous ribosome engineering in E. coli.Nature communications · 2021Article
- Accelerated evolution of a minimal 63-amino acid dual transcription factor.Science advances · 2020Article
- Engineering a Circular Riboregulator inBiodesign research · 2020Article
- Plasmid Characteristics Modulate the Propensity of Gene Exchange in Bacterial Vesicles.Journal of bacteriology · 2019Article
- Spatial and temporal organization of RecA in theeLife · 2019Article
- Engineered promoters enable constant gene expression at any copy number in bacteria.Nature biotechnology · 2018Article
- Isolation and characterization of novel mutations in the pSC101 origin that increase copy number.Scientific reports · 2018Article
- Engineering orthogonal dual transcription factors for multi-input synthetic promoters.Nature communications · 2016Article
- Selection for growth on 3-nitrotoluene by 2-nitrotoluene-utilizing Acidovorax sp. strain JS42 identifies nitroarene dioxygenases with altered specificities.Applied and environmental microbiology · 2015Article
- Tuning the dials of Synthetic Biology.Microbiology (Reading, England) · 2013Review
- Isolation of highly persistent mutants of Salmonella enterica serovar typhimurium reveals a new toxin-antitoxin module.Journal of bacteriology · 2013Article
- Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.Applied and environmental microbiology · 2012Article
- Hapten mediated display and pairing of recombinant antibodies accelerates assay assembly for biothreat countermeasures.Scientific reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE, a structurally similar replication initiator protein from the F factor, the pSC101 repA mutants are predicted to be defective in dimerization. The cloning vectors permit increased expression of gene products along with the advantages of pSC101-derivative plasmids, including stable maintenance and compatibility with ColE1 plasmids. The plasmids also allow blue/white screening for DNA inserts and impart resistance to ampicillin, chloramphenicol and kanamycin. The vectors were used in a genetic assay to suppress temperature-sensitive mutants of ffh, encoding the protein component of the Escherichia coli signal recognition particle, by overproduction of 4.5S RNA. While expression of 4.5S RNA from a wild type pSC101-derivative plasmid was not sufficient for suppression, use of the new vectors did suppress the temperature-sensitive phenotype.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.