ArticleProceedings of the National Academy of Sciences of the United States of America1991
Adhesion of immature thymocytes to thymic stromal cells through fibronectin molecules and its significance for the induction of thymocyte differentiation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Intrathymic Cell Migration: Implications in Thymocyte Development and T Lymphocyte Repertoire Formation.Advances in experimental medicine and biology · 2025Review
- From stem cell to immune effector: how adhesion, migration, and polarity shape T-cell and natural killer cell lymphocyte development in vitro and in vivo.Molecular biology of the cell · 2020Review
- Integrins in T Cell Physiology.International journal of molecular sciences · 2018Review
- The expression of netrin-1 in the thymus and its effects on thymocyte adhesion and migration.Clinical & developmental immunology · 2013Article
- Recipient-derived EDA fibronectin promotes cardiac allograft fibrosis.The Journal of pathology · 2012Article
- Laminin 5 in the human thymus: control of T cell proliferation via alpha6beta4 integrins.The Journal of cell biology · 1999Article
- Enhancement of activation-induced cell death by fibronectin in murine CD4+ CD8+ thymocytes.Immunology · 1998Article
- Epithelial V-like antigen (EVA), a novel member of the immunoglobulin superfamily, expressed in embryonic epithelia with a potential role as homotypic adhesion molecule in thymus histogenesis.The Journal of cell biology · 1998Article
- A novel region of the alpha4 integrin subunit with a modulatory role in VLA-4-mediated cell adhesion to fibronectin.The Biochemical journal · 1997Article
- Impairment of T cell development in deltaEF1 mutant mice.The Journal of experimental medicine · 1997Article
- The vitronectin receptor (alpha V beta 3) as an example for the role of integrins in T lymphocyte stimulation.Immunologic research · 1996Review
- The L1 adhesion molecule is a cellular ligand for VLA-5.The Journal of cell biology · 1995Article
- Extracellular matrix proteins, regulators of T-cell functions in healthy and diseased individuals.Clinical and diagnostic laboratory immunology · 1995Review
- Direct association of pp125FAK with paxillin, the focal adhesion-targeting mechanism of pp125FAK.The Journal of experimental medicine · 1995Article
- Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.The Journal of experimental medicine · 1995Article
- Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells.The Journal of experimental medicine · 1995Article
- Characterization of human thymic epithelial cell surface antigens: phenotypic similarity of thymic epithelial cells to epidermal keratinocytes.Journal of clinical immunology · 1995Article
- Adhesion molecules involved in the binding and subsequent engulfment of thymocytes by a rat thymic epithelial cell line.Immunology · 1994Article
- Prolonged impairment of very late activating antigen-mediated T cell proliferation via the CD3 pathway after T cell-depleted allogeneic bone marrow transplantation.The Journal of clinical investigation · 1994Article
- Induction of programmed cell death in human hematopoietic cell lines by fibronectin via its interaction with very late antigen 5.The Journal of experimental medicine · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Only 10-15% of unseparated thymocytes adhered to culture plates precoated with fibronectin (FN), but 60-70% of the CD4-8- (double-negative) thymocyte population did. This population bound to FN but not to collagen, laminin, or vitronectin. Its binding to FN was inhibited by anti-FN antibody or a mixture of synthetic peptides corresponding to two different sites of FN, termed the V10 sequence and the RGDS (Arg-Gly-Asp-Ser) sequence, which interact, respectively, with the VLA-4 and VLA-5 FN receptors expressed on T-lineage cells. CD4-8- thymocytes also adhered to a monolayer of a thymic stromal cell clone, MRL104.8a, that induces growth-maintenance and differentiation of such thymocytes. The involvement of FN-FN receptor interaction in this adhesion was demonstrated by the following lines of evidence: (i) the MRL104.8a cells expressed FN molecules on their surface and (ii) the adhesion of CD4-8- thymocytes to MRL104.8a monolayers was almost completely inhibited by simultaneous addition of anti-FN antibody and a mixture of peptides (V10 plus RGDS) capable of binding to anti-FN receptors (VLA-4 and -5). Most important, blocking the adhesion of CD4-8- thymocytes to the thymic stromal cell monolayer resulted in potent inhibition of the differentiation of these thymocytes, which was otherwise induced toward the expression of CD4 and/or CD8 molecules. These results indicate that immature CD4-8- thymocytes adhere to thymic stromal cells preferentially through FN-FN receptor interaction and that such adhesion has a critical role in inducing and/or supporting the differentiation of these thymocytes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.