ArticleBMC systems biology2008
Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility.
Article in BMC systems biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
105 citing papers in PubMed, 1 synthesis or guideline pooled it, 282 citations in OpenAlex.
- Genetic mechanisms underlying spermatic and testicular traits within and among cattle breeds: systematic review and prioritization of GWAS results.Journal of animal science · 2018Pooled it
- Influence of sire fertility on the metabolism of in vitro produced embryos.Reproduction & fertility · 2026Article
- Comprehensive proteomics analysis of bovine sperm head plasma membrane associated with fertility.Scientific reports · 2026Article
- Mining public mass spectrometry data to map the bull sperm membrane-associated proteome.Reproduction & fertility · 2026Article
- Morphological and proteomic characterization of sperm in a beluga whale (Delphinapterus leucas):a case report.Veterinary research communications · 2026Article
- High-throughput proteomic profiling reveals dysregulated serum proteins in low fertility bulls linked to nutrient-autophagy-inflammation-extracellular matrix axis.Veterinary research communications · 2025Article
- Precursor A-Kinase Anchor Protein 4 as a Predictive Biomarker of Post-Thaw Semen Quality in Goats.Veterinary sciences · 2025Article
- An Overview of Advancements in Proteomic Approaches to Enhance Livestock Production and Aquaculture.Animals : an open access journal from MDPI · 2025Review
- Electrophoretic protein profiles in seminal plasma and sperm: a comparative study of Bali and Wagyu Bulls.Open veterinary journal · 2025Article
- Stability over Time of the Sperm Motility Biomarker proAKAP4 in Repeated Dog Ejaculates.Animals : an open access journal from MDPI · 2025Article
- Decoding bull fertility in vitro: a proteomics exploration from sperm to blastocyst.Reproduction (Cambridge, England) · 2025Article
- Comparative gene expression profiling of fertility related transcripts in bull sperm of different genetic backgrounds.Tropical animal health and production · 2025Article
- Selecting sires to improve reproductive success: key traits for enhanced fertility and embryo development.Animal reproduction · 2025Article
- Calcium Channels and Modulators as Potential Therapeutic Targets for Contraceptives and Male Fertility: A Scoping Review.Open access journal of contraception · 2025Review
- Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry andAnimal biotechnology · 2024Article
- Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing.Veterinary sciences · 2024Article
- Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly†.Biology of reproduction · 2024Review
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- Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull.Frontiers in veterinary science · 2024Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMale infertility is a major problem for mammalian reproduction. However, molecular details including the underlying mechanisms of male fertility are still not known. A thorough understanding of these mechanisms is essential for obtaining consistently high reproductive efficiency and to ensure lower cost and time-loss by breeder.
resultsUsing high and low fertility bull spermatozoa, here we employed differential detergent fractionation multidimensional protein identification technology (DDF-Mud PIT) and identified 125 putative biomarkers of fertility. We next used quantitative Systems Biology modeling and canonical protein interaction pathways and networks to show that high fertility spermatozoa differ from low fertility spermatozoa in four main ways. Compared to sperm from low fertility bulls, sperm from high fertility bulls have higher expression of proteins involved in: energy metabolism, cell communication, spermatogenesis, and cell motility. Our data also suggests a hypothesis that low fertility sperm DNA integrity may be compromised because cell cycle: G2/M DNA damage checkpoint regulation was most significant signaling pathway identified in low fertility spermatozoa.
conclusionThis is the first comprehensive description of the bovine spermatozoa proteome. Comparative proteomic analysis of high fertility and low fertility bulls, in the context of protein interaction networks identified putative molecular markers associated with high fertility phenotype.
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Registered trials
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