Evidence map›Paper›PMID 18294070›Full record

ArticleHybridoma (2005)2008

Construction of recombinant mouse IgG1 antibody directed against varicella zoster virus immediate early protein 63.

Niklaus H Mueller, Laurie L Graf, Andrew J Shearer, Gregory P Owens, Donald H Gilden, Randall J Cohrs

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hybridoma (2005), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Niklaus H MuellerDepartment of Neurology, University of Colorado School of Medicine, Denver, Colorado 80262, USA.
Laurie L Graf
Andrew J Shearer
Gregory P Owens
Donald H Gilden
Randall J Cohrs
University of Colorado Denver · US

Funding

Varicella Zoster Virus Latency in Human GangliaP01NS032623 · NINDS · UNIVERSITY OF COLORADO DENVER · PI MAHLAINGAM, RAVI · 1993 to 2008
$12.3M
NEUROBIOLOGY OF VARICELLA-ZOSTER VIRUSR01AG006127 · NIA · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD · 1987 to 2014
$4.2M
NEUROVIROLOGY-MOLECULAR BIOLOGYT32NS007321 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD · 1988 to 2014
$2.3M
NEUROBIOLOGY OF VARICELLA ZOSTER VIRUSR37AG006127 · NIA · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD H · 1995 to 2004
$2.3M
NIA NIH HHS AG 06127NIA NIH HHS R01 AG006127NIA NIH HHS R37 AG006127NINDS NIH HHS NS 07321NINDS NIH HHS NS 32623NINDS NIH HHS P01 NS032623NINDS NIH HHS T32 NS007321
6 · The paper itself

Abstract

Five varicella zoster virus (VZV) genes are known to be transcribed in latently infected human ganglia. Transcripts from VZV gene 63, which encodes an immediate early (IE) protein, are the most prevalent and abundant. To obtain a reagent that might facilitate studies of the role of the IE63 protein in latency and reactivation, we selected an IE63-specific Fab fragment from a phage library and used it to prepare a recombinant mouse IgG1 antibody that detects IE63 and functions in Western blot, immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assays.

Indexed as

AnimalsAntigens, ViralCell LineChickenpoxGenes, ViralHerpesvirus 3, HumanHumansImmunoglobulin GMicePeptide LibraryRecombinant ProteinsAntigens, ViralImmunoglobulin GPeptide LibraryRecombinant Proteins

Identifiers

PMID18294070
PMCPMC2938744
OpenAlexW2040798037

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.