ArticleCurrent microbiology2008
The shf gene of a Shigella flexneri homologue on the virulent plasmid pAA2 of enteroaggregative Escherichia coli 042 is required for firm biofilm formation.
Article in Current microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- EnteroaggregativeEcoSal Plus · 2025Review
- Article
- The genomic epidemiology of shigellosis in South Africa.Nature communications · 2023Article
- Article
- Inactivation of theMicroorganisms · 2020Article
- Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease.Scientific reports · 2020Article
- Shiga Toxin-Producing and Enteroaggregative Escherichia coli in Animal, Foods, and Humans: Pathogenicity Mechanisms, Detection Methods, and Epidemiology.Current microbiology · 2020Review
- Tracing Back the Evolutionary Route of EnteroinvasiveFrontiers in cellular and infection microbiology · 2020Article
- Genetic and Virulence Profiles of EnteroaggregativeFrontiers in cellular and infection microbiology · 2020Article
- Organization and architecture of AggR-dependent promoters from enteroaggregative Escherichia coli.Molecular microbiology · 2019Article
- Draft Genome Sequence ofGenome announcements · 2018Article
- pic gene of enteroaggregative Escherichia coli and its association with diarrhea in Peruvian children.Pathogens and disease · 2016Article
- Epidemiology and clinical manifestations of enteroaggregative Escherichia coli.Clinical microbiology reviews · 2014Review
- EnteroaggregativeCurrent tropical medicine reports · 2014Article
- Prevalence of enteroaggregative Escherichia coli and its virulence-related genes in a case-control study among children from north-eastern Brazil.Journal of medical microbiology · 2013Article
- The heat-resistant agglutinin family includes a novel adhesin from enteroaggregative Escherichia coli strain 60A.Journal of bacteriology · 2011Article
- A pathoadaptive deletion in an enteroaggregative Escherichia coli outbreak strain enhances virulence in a Caenorhabditis elegans model.Infection and immunity · 2010Article
- Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.PloS one · 2010Article
- Enteroaggregative Escherichia coli: An Emerging Enteric Food Borne Pathogen.Interdisciplinary perspectives on infectious diseases · 2010Article
- Article
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6 authors at 1 institution in 1 country.
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Abstract
Enteroaggregative Escherichia coli (EAEC) is an increasingly important cause of diarrhea in both developing and industrialized countries, and is characterized by strong biofilm formation on the intestinal mucosa. Sequencing of the virulent plasmid pAA2 of the prototype EAEC 042 revealed a cluster of three open reading frames (ORFs; shf, capU, and virK) ca. 93% identical to a similar cluster located in Shigella flexneri. The function of the first ORF Shf protein is not known, but the closest well-characterized homologue is the IcaB protein of Staphylococcus epidermidis, which plays a crucial role in exopolysaccharide modification in bacterial biofilm formation. To investigate the role of this cluster in the virulence of EAEC, we mutated three genes at this locus. All the mutants maintained the aggregative phenotype in the liquid phase. However, the insertional mutant of shf formed a less abundant biofilm in a microtiter plate assay than did the wild type, while the capU mutant and the virK mutant did not. The complementation of the shf mutant with this cluster restored the thick biofilm similar to that of the wild type. The shf transcriptional level decreased in the transcriptional regulator aggR mutant and was restored when the mutant was complemented with aggR. These results suggest that the shf gene is required for the firm biofilm formation of EAEC 042, and transcription of the shf gene is dependent on AggR.
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