ArticleJournal of molecular biology2008
Methanothermobacter thermautotrophicus tRNA Gln confines the amidotransferase GatCAB to asparaginyl-tRNA Asn formation.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Unconventional genetic code systems in archaea.Frontiers in microbiology · 2022Review
- Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Drosophila nuclear factor DREF regulates the expression of the mitochondrial DNA helicase and mitochondrial transcription factor B2 but not the mitochondrial translation factor B1.Biochimica et biophysica acta · 2013Article
- Insights into archaeal evolution and symbiosis from the genomes of a nanoarchaeon and its inferred crenarchaeal host from Obsidian Pool, Yellowstone National Park.Biology direct · 2013Article
- The asparagine-transamidosome from Helicobacter pylori: a dual-kinetic mode in non-discriminating aspartyl-tRNA synthetase safeguards the genetic code.Nucleic acids research · 2012Article
- Two-step aminoacylation of tRNA without channeling in Archaea.Journal of molecular biology · 2011Article
- The archaeal transamidosome for RNA-dependent glutamine biosynthesis.Nucleic acids research · 2010Article
- Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition.Nucleic acids research · 2010Article
- Insights into tRNA-dependent amidotransferase evolution and catalysis from the structure of the Aquifex aeolicus enzyme.Journal of molecular biology · 2009Article
- Amino acid modifications on tRNA.Acta biochimica et biophysica Sinica · 2008Review
- From one amino acid to another: tRNA-dependent amino acid biosynthesis.Nucleic acids research · 2008Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Many prokaryotes form the amide aminoacyl-tRNAs glutaminyl-tRNA and asparaginyl-tRNA by tRNA-dependent amidation of the mischarged tRNA species, glutamyl-tRNA(Gln) or aspartyl-tRNA(Asn). Archaea employ two such amidotransferases, GatCAB and GatDE, while bacteria possess only one, GatCAB. The Methanothermobacter thermautotrophicus GatDE is slightly more efficient using Asn as an amide donor than Gln (k(cat)/K(M) of 5.4 s(-1)/mM and 1.2 s(-1)/mM, respectively). Unlike the bacterial GatCAB enzymes studied to date, the M. thermautotrophicus GatCAB uses Asn almost as well as Gln as an amide donor (k(cat)/K(M) of 5.7 s(-1)/mM and 16.7 s(-1)/mM, respectively). In contrast to the initial characterization of the M. thermautotrophicus GatCAB as being able to form Asn-tRNA(Asn) and Gln-tRNA(Gln), our data demonstrate that while the enzyme is able to transamidate Asp-tRNA(Asn) (k(cat)/K(M) of 125 s(-1)/mM) it is unable to transamidate M. thermautotrophicus Glu-tRNA(Gln). However, M. thermautotrophicus GatCAB is capable of transamidating Glu-tRNA(Gln) from H. pylori or B. subtilis, and M. thermautotrophicus Glu-tRNA(Asn). Thus, M. thermautotrophicus encodes two amidotransferases, each with its own activity, GatDE for Gln-tRNA and GatCAB for Asn-tRNA synthesis.
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