ArticleMolecular biology of the cell2008
Ste20-related protein kinase LOSK (SLK) controls microtubule radial array in interphase.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Golgi-Associated Protein JAKMIP2 Is Linked to the Centrosome and Performs Microtubule-Related Functions.Cells · 2025Article
- Centrosome-derived microtubule radial array, PCM-1 protein, and primary cilia formation.Protoplasma · 2019Article
- Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness.Skeletal muscle · 2017Article
- Regulation of Catalytic and Non-catalytic Functions of the Drosophila Ste20 Kinase Slik by Activation Segment Phosphorylation.The Journal of biological chemistry · 2015Article
- Ste20-like protein kinase SLK (LOSK) regulates microtubule organization by targeting dynactin to the centrosome.Molecular biology of the cell · 2013Article
- Distinct roles for Ste20-like kinase SLK in muscle function and regeneration.Skeletal muscle · 2013Article
- FAM123A binds to microtubules and inhibits the guanine nucleotide exchange factor ARHGEF2 to decrease actomyosin contractility.Science signaling · 2012Article
- Regulation of the Ste20-like kinase, SLK: involvement of activation segment phosphorylation.The Journal of biological chemistry · 2012Article
- Activity of the Ste20-like kinase, SLK, is enhanced by homodimerization.American journal of physiology. Renal physiology · 2011Article
- Prostate-derived sterile 20-like kinases (PSKs/TAOKs) are activated in mitosis and contribute to mitotic cell rounding and spindle positioning.The Journal of biological chemistry · 2011Article
- The Golgi and the centrosome: building a functional partnership.The Journal of cell biology · 2010Review
- Bioinformatic search of plant microtubule-and cell cycle related serine-threonine protein kinases.BMC genomics · 2010Article
- The mammalian family of sterile 20p-like protein kinases.Pflugers Archiv : European journal of physiology · 2009Review
- Article
- LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Ste20-like kinase SLK, at the crossroads: a matter of life and death.Cell adhesion & migrationReview
- A novel role for the Ste20 kinase SLK in adhesion signaling and cell migration.Cell adhesion & migrationReview
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interphase microtubules are organized into a radial array with centrosome in the center. This organization is a subject of cellular regulation that can be driven by protein phosphorylation. Only few protein kinases that regulate microtubule array in interphase cells have been described. Ste20-like protein kinase LOSK (SLK) was identified as a microtubule and centrosome-associated protein. In this study we have shown that the inhibition of LOSK activity by dominant-negative mutant K63R-DeltaT or by LOSK depletion with RNAi leads to unfocused microtubule arrangement. Microtubule disorganization is prominent in Vero, CV-1, and CHO-K1 cells but less distinct in HeLa cells. The effect is a result neither of microtubule stabilization nor of centrosome disruption. In cells with suppressed LOSK activity centrosomes are unable to anchor or to cap microtubules, though they keep nucleating microtubules. These centrosomes are depleted of dynactin. Vero cells overexpressing K63R-DeltaT have normal dynactin "comets" at microtubule ends and unaltered morphology of Golgi complex but are unable to polarize it at the wound edge. We conclude that protein kinase LOSK is required for radial microtubule organization and for the proper localization of Golgi complex in various cell types.
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