ArticleMolecular biology of the cell2008
Cell cycle-dependent binding of HMGN proteins to chromatin.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 1 citations in OpenAlex.
- Shaking up the silence: consequences of HMGN1 antagonizing PRC2 in the Down syndrome brain.Epigenetics & chromatin · 2022Review
- Peritoneal Carcinomatosis Targeting with Tumor Homing Peptides.Molecules (Basel, Switzerland) · 2018Review
- Functional compensation among HMGN variants modulates the DNase I hypersensitive sites at enhancers.Genome research · 2015Article
- Cbx2 stably associates with mitotic chromosomes via a PRC2- or PRC1-independent mechanism and is needed for recruiting PRC1 complex to mitotic chromosomes.Molecular biology of the cell · 2014Article
- Profiling of the Chromatin-associated Proteome Identifies HP1BP3 as a Novel Regulator of Cell Cycle Progression.Molecular & cellular proteomics : MCP · 2014Article
- The chromatin architectural proteins HMGD1 and H1 bind reciprocally and have opposite effects on chromatin structure and gene regulation.BMC genomics · 2014Article
- A multi-resource data integration approach: identification of candidate genes regulating cell proliferation during neocortical development.Frontiers in neuroscience · 2014Article
- HMGN1 modulates nucleosome occupancy and DNase I hypersensitivity at the CpG island promoters of embryonic stem cells.Molecular and cellular biology · 2013Article
- The HMGN family of chromatin-binding proteins: dynamic modulators of epigenetic processes.Biochimica et biophysica acta · 2012Review
- Development of a chemical genetic approach for human aurora B kinase identifies novel substrates of the chromosomal passenger complex.Molecular & cellular proteomics : MCP · 2012Article
- Divalent metal- and high mobility group N protein-dependent nucleosome stability and conformation.Journal of nucleic acids · 2010Article
- Downregulation of the nucleosome-binding protein 1 (NSBP1) gene can inhibit the in vitro and in vivo proliferation of prostate cancer cells.Asian journal of andrology · 2010Article
- The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA.The Journal of biological chemistry · 2010Article
- Article
- The dynamics of HMG protein-chromatin interactions in living cells.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2009Review
- Article
- Differential expression of the HMGN family of chromatin proteins during ocular development.Gene expression patterns : GEP · 2008Article
- Nuclear functions of the HMG proteins.Biochimica et biophysica actaReview
- Regulation of chromatin structure and function by HMGN proteins.Biochimica et biophysica actaReview
- HMGN5/NSBP1: a new member of the HMGN protein family that affects chromatin structure and function.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Throughout the cell cycle, the histones remain associated with DNA, but the repertoire of proteins associated with the chromatin fiber continuously changes. The chromatin interaction of HMGNs, a family of nucleosome binding proteins that modulates the structure and activity of chromatin, during the cell cycle is controversial. Immunofluorescence studies demonstrated that HMGNs are not associated with chromatin, whereas live cell imaging indicated that they are present in mitotic chromosomes. To resolve this controversy, we examined the organization of wild-type and mutated HMGN1 and HMGN2 proteins in the cell nucleus by using immunofluorescence studies, live cell imaging, gel mobility shift assays, and bimolecular fluorescence complementation (BiFC). We find that during interphase, HMGNs bind specifically to nucleosomes and form homodimeric complexes that yield distinct BiFC signals. In metaphase, the nucleosomal binding domain of the protein is inactivated, and the proteins associate with chromatin with low affinity as monomers, and they do not form specific complexes. Our studies demonstrate that the mode of binding of HMGNs to chromatin is cell cycle dependent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.