ArticleEuropean biophysics journal : EBJ2008
Solvent isotope effect on macromolecular dynamics in E. coli.
Article in European biophysics journal : EBJ, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Proteome-wide 3D structure prediction provides insights into the ancestral metabolism of ancient archaea and bacteria.Nature communications · 2022Article
- The impact of folding modes and deuteration on the atomic resolution structure of hen egg-white lysozyme.Acta crystallographica. Section D, Structural biology · 2021Article
- Article
- Thermophoresis: The Case of Streptavidin and Biotin.Polymers · 2020Article
- Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments.Nature protocols · 2016Article
- Impact of Deuteration on the Assembly Kinetics of Transthyretin Monitored by Native Mass Spectrometry and Implications for Amyloidoses.Angewandte Chemie (International ed. in English) · 2016Article
- Solution properties of γ-crystallins: hydration of fish and mammal γ-crystallins.Protein science : a publication of the Protein Society · 2014Article
- Straight lines of neutron scattering in biology: a review of basic controls in SANS and EINS.European biophysics journal : EBJ · 2012Review
- Dynamics-stability relationships in apo- and holomyoglobin: a combined neutron scattering and molecular dynamics simulations study.Biophysical journal · 2012Article
- Article
- Dynamics of the cytoskeleton: how much does water matter?Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Atomic-scale dynamics inside living cells explored by neutron scattering.Journal of the Royal Society, Interface · 2009Review
- Hemoglobin senses body temperature.European biophysics journal : EBJ · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elastic incoherent neutron scattering was used to explore solvent isotope effects on average macromolecular dynamics in vivo. Measurements were performed on living E. coli bacteria containing H2O and D2O, respectively, close to physiological conditions of temperature. Global macromolecular flexibility, expressed as mean square fluctuation (MSF) values, and structural resilience in a free energy potential, expressed as a mean effective force constant, [Symbol: see text]k'[Symbol: see text], were extracted in the two solvent conditions. They referred to the average contribution of all macromolecules inside the cell, mostly dominated by the internal motions of the protein fraction. Flexibility and resilience were both found to be smaller in D2O than in H2O. A difference was expected because the driving forces behind macromolecular stabilization and dynamics are different in H2O and D2O. In D2O, the hydrophobic effect is known to be stronger than in H2O: it favours the burial of non-polar surfaces as well as their van der Waals' packing in the macromolecule cores. This may lead to the observed smaller MSF values. In contrast, in H2O, macromolecules would present more water-exposed surfaces, which would give rise to larger MSF values, in particular at the macromolecular surface. The smaller [Symbol: see text]k'[Symbol: see text] value suggested a larger entropy content in the D2O case due to increased sampling of macromolecular conformational substates.
Indexed as
Identifiers
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Registered trials
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