ArticlePloS one2008
The leishmania ARL-1 and Golgi traffic.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Roles of ADP-Ribosylation during Infection Establishment byPathogens (Basel, Switzerland) · 2023Review
- Comparative Proteomics and Genome-Wide Druggability Analyses Prioritized Promising Therapeutic Targets against Drug-ResistantMicroorganisms · 2023Article
- Drug discovery in leishmaniasis using protein lipidation as a target.Biophysical reviews · 2021Review
- Novel Thienopyrimidine Inhibitors ofJournal of medicinal chemistry · 2020Article
- Deciphering the role of UBA-like domains in intraflagellar distribution and functions of myosin XXI in Leishmania.PloS one · 2020Article
- Recombinant protein ofOncotarget · 2017Article
- Global analysis of protein N-myristoylation and exploration of N-myristoyltransferase as a drug target in the neglected human pathogen Leishmania donovani.Chemistry & biology · 2015Article
- Acylation in trypanosomatids: an essential process and potential drug target.Trends in parasitology · 2014Review
- Golgi traffic and integrity depend on N-myristoyl transferase-1 in Arabidopsis.The Plant cell · 2013Article
- Roles of N-terminal fatty acid acylations in membrane compartment partitioning: Arabidopsis h-type thioredoxins as a case study.The Plant cell · 2013Article
- Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.The Journal of biological chemistry · 2012Article
- Overexpression of S4D mutant of Leishmania donovani ADF/cofilin impairs flagellum assembly by affecting actin dynamics.Eukaryotic cell · 2012Article
- Evolutionary and functional insights into Leishmania META1: evidence for lateral gene transfer and a role for META1 in secretion.BMC evolutionary biology · 2011Article
- Crystal structure of Leishmania major ADP-ribosylation factor-like 1 and a classification of related GTPase family members in this Kinetoplastid.Molecular and biochemical parasitology · 2010Article
- The role of acidocalcisomes in parasitic protists.The Journal of eukaryotic microbiologyReview
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present here the characterisation of the Leishmania small G protein ADP-Ribosylation Factor-Like protein 1 (ARL-1). The ARL-1 gene is present in one copy per haploid genome and conserved among trypanosomatids. It encodes a protein of 20 kDa, which is equally expressed in the insect promastigote and mammalian amastigote forms of the parasite. ARL-1 localises to the Trans-Golgi Network (TGN); N-terminal myristoylation is essential for TGN localisation. In vivo expression of the LdARL-1/Q74L and LdARL-1/T51N mutants (GTP- and GDP-bound blocked forms respectively) shows that GDP/GTP cycling occurs entirely within the TGN. This is contrary to previous reports in yeast and mammals, where the mutant empty form devoid of nucleotide has been considered as the GDP-blocked form. The dominant-negative empty form mutant LdARL-1/T34N inhibits endocytosis and intracellular trafficking from the TGN to the Lysosome/Multivesicular Tubule and to the acidocalcisomes; these defects are probably related to a mislocalisation of the GRIP domain-containing vesicle tethering factors which cannot be recruited to the TGN by the cytoplasmic LdARL-1/T34N. Thus, besides the functional characterization of a new mutant and a better understanding of ARL-1 GDP/GTP cycling, this work shows that Leishmania ARL-1 is a key component of an essential pathway worth future study.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.