ArticleMolecular and cellular biology2008
Splicing factors SF1 and U2AF associate in extraspliceosomal complexes.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- U2AF2 controls alternative splicing in speckle-proximal regions in an RS domain-dependent manner.Nucleic acids research · 2026Article
- A deep learning model for characterizing protein-RNA interactions from sequences at single-base resolution.Patterns (New York, N.Y.) · 2025Article
- Proximity labeling defines the phagosome lumen proteome of murine and primary human macrophages.bioRxiv : the preprint server for biology · 2024Article
- An axonemal intron splicing program sustains Plasmodium male development.Nature communications · 2024Article
- Commitment Complex Splicing Factors in Cancers of the Gastrointestinal Tract-An In Silico Study.Bioinformatics and biology insights · 2024Article
- The distinct roles of zinc finger CCHC-type (ZCCHC) superfamily proteins in the regulation of RNA metabolism.RNA biology · 2021Review
- SRSF5 regulates alternative splicing ofRNA biology · 2021Article
- KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.G3 (Bethesda, Md.) · 2021Article
- TGIF1 and SF1 polymorphisms are associated with litter size in Small Tail Han sheep.Reproduction in domestic animals = Zuchthygiene · 2020Article
- Optimization of a Bioluminescence Resonance Energy Transfer-Based Assay for Screening of Trypanosoma cruzi Protein/Protein Interaction Inhibitors.Molecular biotechnology · 2018Article
- The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms.Plant cell reports · 2017Article
- SF1 Phosphorylation Enhances Specific Binding to U2AFBiophysical journal · 2016Article
- The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing.RNA (New York, N.Y.) · 2016Article
- Discovery of prognostic biomarkers for predicting lung cancer metastasis using microarray and survival data.BMC bioinformatics · 2015Article
- Rbm20-deficient cardiogenesis reveals early disruption of RNA processing and sarcomere remodeling establishing a developmental etiology for dilated cardiomyopathy.Human molecular genetics · 2014Article
- Two splicing factors carrying serine-arginine motifs, TSR1 and TSR1IP, regulate splicing, mRNA stability, and rRNA processing in Trypanosoma brucei.RNA biology · 2014Article
- Basal splicing factors regulate the stability of mature mRNAs in trypanosomes.The Journal of biological chemistry · 2013Article
- Probing nucleic acid interactions and pre-mRNA splicing by Förster Resonance Energy Transfer (FRET) microscopy.International journal of molecular sciences · 2012Review
- Intron definition and a branch site adenosine at nt 385 control RNA splicing of HPV16 E6*I and E7 expression.PloS one · 2012Article
- Branch point identification and sequence requirements for intron splicing in Plasmodium falciparum.Eukaryotic cell · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Splicing factors SF1 and U2AF associate cooperatively with pre-mRNA and play a crucial role in 3' splice site recognition during early steps of spliceosome assembly. Formation of the active spliceosome subsequently displaces SF1 in a remodeling process that stabilizes the association of U2 snRNP with pre-mRNA. Fluorescence microscopy shows SF1 and U2AF distributed throughout the nucleoplasm, where transcription occurs, with additional concentration in nuclear speckles, where splicing factors accumulate when not engaged in splicing. Fluorescence recovery after photobleaching analysis in live cells shows that the mobilities of SF1 and the two subunits of U2AF (U2AF(65) and U2AF(35)) are correlated with the abilities of these proteins to interact with each other. Direct binding of SF1 to U2AF(65) was demonstrated by fluorescence resonance energy transfer in both the nucleoplasm and nuclear speckles. This interaction persisted after transcription inhibition, suggesting that SF1 associates with U2AF in a splicing-independent manner. We propose that SF1 and U2AF form extraspliceosomal complexes before and after taking part in the assembly of catalytic spliceosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.