ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2008
Murine mCLCA6 is an integral apical membrane protein of non-goblet cell enterocytes and co-localizes with the cystic fibrosis transmembrane conductance regulator.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- The impact of disease and species differences on the intestinal CLCA4 gene expression.Journal of molecular medicine (Berlin, Germany) · 2025Article
- Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure.Cell discovery · 2023Article
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- Naturally Occurring Deletion Mutants of the Pig-Specific, Intestinal Crypt Epithelial Cell Protein CLCA4b without Apparent Phenotype.PloS one · 2015Article
- Self-cleavage of human CLCA1 protein by a novel internal metalloprotease domain controls calcium-activated chloride channel activation.The Journal of biological chemistry · 2012Article
- IL-13-induced airway mucus production is attenuated by MAPK13 inhibition.The Journal of clinical investigation · 2012Article
- Impaired autoproteolytic cleavage of mCLCA6, a murine integral membrane protein expressed in enterocytes, leads to cleavage at the plasma membrane instead of the endoplasmic reticulum.Molecules and cells · 2012Article
- The porcine chloride channel calcium-activated family member pCLCA4a mirrors lung expression of the human hCLCA4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- The murine goblet cell protein mCLCA3 is a zinc-dependent metalloprotease with autoproteolytic activity.Molecules and cells · 2011Article
- Cellular distribution and subcellular localization of mCLCA1/2 in murine gastrointestinal epithelia.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Murine mCLCA5 is expressed in granular layer keratinocytes of stratified epithelia.Histochemistry and cell biology · 2010Article
- Cftr and ENaC ion channels mediate NaCl absorption in the mouse submandibular gland.The Journal of physiology · 2010Article
- Genomic, tissue expression, and protein characterization of pCLCA1, a putative modulator of cystic fibrosis in the pig.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- hCLCA2 Is a p53-Inducible Inhibitor of Breast Cancer Cell Proliferation.Cancer research · 2009Article
- The role of CLCA proteins in inflammatory airway disease.Annual review of physiology · 2009Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CLCA family of proteins consists of a growing number of structurally and functionally diverse members with distinct expression patterns in different tissues. Several CLCA homologs have been implicated in diseases with secretory dysfunctions in the respiratory and intestinal tracts. Here we present biochemical protein characterization and details on the cellular and subcellular expression pattern of the murine mCLCA6 using specific antibodies directed against the amino- and carboxy-terminal cleavage products of mCLCA6. Computational and biochemical characterizations revealed protein processing and structural elements shared with hCLCA2 including anchorage in the apical cell membrane by a transmembrane domain in the carboxy-terminal subunit. A systematic light- and electron-microscopic immunolocalization found mCLCA6 to be associated with the microvilli of non-goblet cell enterocytes in the murine small and large intestine but in no other tissues. The expression pattern was confirmed by quantitative RT-PCR following laser-capture microdissection of relevant tissues. Confocal laser scanning microscopy colocalized the mCLCA6 protein with the cystic fibrosis transmembrane conductance regulator CFTR at the apical surface of colonic crypt cells. Together with previously published functional data, the results support a direct or indirect role of mCLCA6 in transepithelial anion conductance in the mouse intestine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.