ArticleBMC developmental biology2008
Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta.
Article in BMC developmental biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 90 citations in OpenAlex.
- Optimization of a Bovine Intracytoplasmic Sperm Injection System Using Sex-Sorted Sperm: Screening with Slaughterhouse-Derived Oocytes and Validation with Ovum Pick-Up-Derived Oocytes.Animals : an open access journal from MDPI · 2026Article
- Genomic Insights into Fertilization: Tracing PLCZ1 Orthologs Across Amphibian Lineages.Genome biology and evolution · 2025Article
- An advanced cytosine base editor enabled the generation of cattle with a stop codon in the β-lactoglobulin gene.Transgenic research · 2025Article
- Phospholipase C Zeta 1 (PLCZ1): The Function and Potential for Fertility Assessment and In Vitro Embryo Production in Cattle and Horses.Veterinary sciences · 2023Review
- Article
- SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1.Reproduction (Cambridge, England) · 2022Article
- Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation.Cell and tissue research · 2021Article
- Intracytoplasmic Sperm Injection in Cattle.Genes · 2021Review
- Dysregulated Gene Expression of Imprinted and X-Linked Genes: A Link to Poor Development of Bovine Haploid Androgenetic Embryos.Frontiers in cell and developmental biology · 2021Article
- Bovine eggs release zinc in response to parthenogenetic and sperm-induced egg activation.Theriogenology · 2019Article
- PLCζ Induced CaFrontiers in cell and developmental biology · 2018Article
- Calcium influx and sperm-evoked calcium responses during oocyte maturation and egg activation.Oncotarget · 2017Review
- Active H3K27me3 demethylation by KDM6B is required for normal development of bovine preimplantation embryos.Epigenetics · 2017Article
- Lack of calcium oscillation causes failure of oocyte activation after intracytoplasmic sperm injection in pigs.The Journal of reproduction and development · 2016Article
- Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes.Journal of visualized experiments : JoVE · 2016Article
- PLCζ sequence, protein levels, and distribution in human sperm do not correlate with semen characteristics and fertilization rates after ICSI.Journal of assisted reproduction and genetics · 2016Article
- Vertebrate Reproduction.Cold Spring Harbor perspectives in biology · 2015Review
- DPPA3 prevents cytosine hydroxymethylation of the maternal pronucleus and is required for normal development in bovine embryos.Epigenetics · 2014Article
- Suppression of chemically induced and spontaneous mouse oocyte activation by AMP-activated protein kinase.Biology of reproduction · 2013Article
- Oocyte activation and phospholipase C zeta (PLCζ): diagnostic and therapeutic implications for assisted reproductive technology.Cell communication and signaling : CCS · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundDuring natural fertilization, sperm fusion with the oocyte induces long lasting intracellular calcium oscillations which in turn are responsible for oocyte activation. PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response. We tested the hypothesis that PLCZ1 cRNA injection can be used to activate bovine oocytes.
resultsMouse and bovine PLCZ1 cRNAs were injected into matured bovine oocytes at different concentrations. Within the concentrations tested, mouse PLCZ1 injection activated bovine oocytes at a maximum rate when the pipette concentration of cRNA ranged from 0.25 to 1 mug/muL, while bovine PLCZ1 was optimal at 0.1 mug/muL. At their most effective concentrations, PLCZ1 induced parthenogenetic development at rates similar to those observed using other activation stimuli such as Ionomycin/CHX and Ionomycin/DMAP. Injection of mouse and bovine PLCZ1 cRNA induced dose-dependent sperm-like calcium oscillations whose frequency increased over time. Injection of bovine and mouse PLCZ1 cRNA also induced IP3R-1 degradation, although bovine PLCZ1 cRNA evoked greater receptor degradation than its mouse counterpart.
conclusionInjection of PLCZ1 cRNA efficiently activated bovine oocytes by inducing a sperm-like calcium oscillatory pattern. Importantly, the high rate of aneuploidy encountered in parthenogenetic embryos activated by certain chemical means was not observed in PLCZ1 activated embryos.
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Registered trials
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