ArticleNucleic acids research1991
NF-I proteins from brain interact with the proenkephalin cAMP inducible enhancer.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 44 citations in OpenAlex.
- A genome-wide screen of CREB occupancy identifies the RhoA inhibitors Par6C and Rnd3 as regulators of BDNF-induced synaptogenesis.PloS one · 2013Article
- Nuclear factor 1 is required for both hormone-dependent chromatin remodeling and transcriptional activation of the mouse mammary tumor virus promoter.Molecular and cellular biology · 2003Article
- Second-site proviral enhancer alterations in lymphomas induced by enhancer mutants of SL3-3 murine leukemia virus: negative effect of nuclear factor 1 binding site.Journal of virology · 1997Article
- The transcriptional regulation of the preproenkephalin gene.The Biochemical journal · 1995Review
- Fibroblast growth factor and cyclic AMP (cAMP) synergistically activate gene expression at a cAMP response element.Molecular and cellular biology · 1994Article
- Cloning and functional analysis of spliced isoforms of human nuclear factor I-X: interference with transcriptional activation by NFI/CTF in a cell-type specific manner.Nucleic acids research · 1994Article
- Jun is phosphorylated by several protein kinases at the same sites that are modified in serum-stimulated fibroblasts.Molecular and cellular biology · 1992Article
- Preproenkephalin promoter "cassette" confers brain expression and synaptic regulation in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cell-type-specific expression of the rat thyroperoxidase promoter indicates common mechanisms for thyroid-specific gene expression.Molecular and cellular biology · 1992Article
- Interaction of DNA-binding proteins with a milk protein gene promoter in vitro: identification of a mammary gland-specific factor.Nucleic acids research · 1991Article
- Protein-DNA interactions during phenotypic differentiation.Molecular neurobiologyArticle
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A short region of the human proenkephalin promoter has been shown previously to mediate transcriptional regulation in response to activation of the cAMP, TPA, and Ca+ + dependent intracellular signalling pathways. Two adjacent DNA elements, CRE-1 and CRE-2, are essential for this regulation although neither element alone is sufficient for inducible expression. The CRE-2 element consists of overlapping binding sites for the transcription factors AP-1 and AP-4. The CRE-1 element has been shown to interact with a DNA binding factor called ENKTF-1. Here we characterize proteins from bovine brain which bind the CRE-1 element of the human proenkephalin gene. Interactions between proteins binding the CRE-1 and CRE-2 elements are characterized in vitro using affinity purified DNA binding proteins. We demonstrate that CRE-1 binding proteins from bovine brain consist of three different polypeptides each belonging to the NF-I family of transcription factors. Point mutation analysis of the contacts of these proteins with the CRE-1 element indicate that NF-I proteins contact the inducible enhancer at the sequence CTGGCxxxxxxCCT which overlaps the CRE-1 element (underlined) defined by in vivo point mutation analysis. Cotransfection of one of the three NF-I proteins purified from bovine brain, NF-I/Red1, together with a proenkephalin/bacterial chloramphenicol acetyl transferase (CAT) fusion gene repressed protein kinase A or forskolin stimulated CAT expression.
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