ArticleBMC genomics2008
Multiplex-ready PCR: a new method for multiplexed SSR and SNP genotyping.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed.
- Development and Application of an SNP Marker for High-Throughput Detection and Utilization of theGenes · 2025Article
- Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information.Communications biology · 2025Article
- Genebank Management Through Microsatellite Markers: A Case Study in Two Italian Peach Germplasm Collections.Plants (Basel, Switzerland) · 2025Article
- SSR_VibraProfiler: a Python package for accurate classification of varieties using SSRs with intra-variety specificity and inter-variety polymorphism.Plant methods · 2025Article
- Optimization of Breeding Tools in Quinoa (Plants (Basel, Switzerland) · 2024Article
- Parental Reconstruction from a Half-Sib Population of Stoneless JujubePlants (Basel, Switzerland) · 2024Article
- Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP).Scientific reports · 2024Article
- SSR-based molecular diagnosis for Taiwan tea cultivars and its application in identifying cultivar composition of the processed tea.Journal of food and drug analysis · 2023Article
- Population Genetics of Manila Clam (Biology · 2023Article
- Probe design for simultaneous, targeted capture of diverse metagenomic targets.Cell reports methods · 2021Article
- Fine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3.Horticulture research · 2020Article
- Ethylene Is Not Essential for R-Gene Mediated Resistance but Negatively Regulates Moderate Resistance to Some Aphids inInternational journal of molecular sciences · 2020Article
- Identification of molecular markers for the Pc39 gene conferring resistance to crown rust in oat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Diverging landscape impacts on macronutrient status despite overlapping diets in managed (Conservation physiology · 2020Article
- Genetic variation of HvXYN1 associated with endoxylanase activity and TAX content in barley (Hordeum vulgare L.).BMC plant biology · 2019Article
- CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system.Nucleic acids research · 2019Article
- Low Amplitude Boom-and-Bust Cycles Define the Septoria Nodorum Blotch Interaction.Frontiers in plant science · 2019Article
- Multiple quantitative trait loci contribute to resistance to bacterial canker incited byHorticulture research · 2019Article
- Development of 17 novel microsatellite markers forApplications in plant sciences · 2018Article
- Development of novel EST-SSR markers forApplications in plant sciences · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrosatellite (SSR) and single nucleotide polymorphism (SNP) markers are widely used in plant breeding and genomic research. Thus, methods to improve the speed and efficiency of SSR and SNP genotyping are highly desirable. Here we describe a new method for multiplex PCR that facilitates fluorescence-based SSR genotyping and the multiplexed preparation of DNA templates for SNP assays.
resultsWe show that multiplex-ready PCR can achieve a high (92%) success rate for the amplification of published sequences under standardised reaction conditions, with a PCR specificity comparable to that of conventional PCR methods. We also demonstrate that multiplex-ready PCR supports an improved level of multiplexing in plant genomes of varying size and ploidy, without the need to carefully optimize assay conditions. Several advantages of multiplex-ready PCR for SSR and SNP genotyping are demonstrated and discussed. These include the uniform amplification of target sequences within multiplexed reactions and between independent assays, and the ability to label amplicons during PCR with specialised moieties such fluorescent dyes and biotin.
conclusionMultiplex-ready PCR provides several technological advantages that can facilitate fluorescence-based SSR genotyping and the multiplexed preparation of DNA templates for SNP assays. These advantages can be captured at several points in the genotyping process, and offer considerable cost and labour savings. Multiplex-ready PCR is broadly applicable to plant genomics and marker assisted breeding, and should be transferable to any animal or plant species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.