ArticleNucleic acids research2008
Phage T4 SegB protein is a homing endonuclease required for the preferred inheritance of T4 tRNA gene region occurring in co-infection with a related phage.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 42 citations in OpenAlex.
- Review
- Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity.Nature communications · 2026Article
- Genomic landscape of completeFrontiers in microbiology · 2026Article
- Post-transcriptional control during T4 infection.EcoSal Plus · 2025Review
- Diversity and functional characterization of HNH endonucleases encoded by lactococcalMicrobial genomics · 2025Article
- Characterizations of Newly IsolatedViruses · 2025Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
- Discovery of Bactericidal Proteins fromAntibiotics (Basel, Switzerland) · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.bioRxiv : the preprint server for biology · 2023Article
- Article
- Isolation of bacteriophages infectingFrontiers in microbiology · 2023Article
- Isolation and Characterization of Novel Lytic Phages Infecting Multidrug-Resistant Escherichia coli.Microbiology spectrum · 2022Article
- Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds.Microbiology spectrum · 2022Article
- Phenotypic and Genomic Comparison ofViruses · 2021Article
- Role of a cryptic tRNA gene operon in survival under translational stress.Nucleic acids research · 2021Article
- Tailed Lytic Bacteriophages of Soft Rot Pectobacteriaceae.Microorganisms · 2021Review
- Comparative genomics of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members emphasizing tRNA and non-coding RNA.BMC evolutionary biology · 2019Article
- Complete Genome Sequence of Klebsiella pneumoniae Myophage Mineola.Microbiology resource announcements · 2019Article
- GlobalViruses · 2019Article
- Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homing endonucleases initiate nonreciprocal transfer of DNA segments containing their own genes and the flanking sequences by cleaving the recipient DNA. Bacteriophage T4 segB gene, which is located in a cluster of tRNA genes, encodes a protein of unknown function, homologous to homing endonucleases of the GIY-YIG family. We demonstrate that SegB protein is a site-specific endonuclease, which produces mostly 3' 2-nt protruding ends at its DNA cleavage site. Analysis of SegB cleavage sites suggests that SegB recognizes a 27-bp sequence. It contains 11-bp conserved sequence, which corresponds to a conserved motif of tRNA TpsiC stem-loop, whereas the remainder of the recognition site is rather degenerate. T4-related phages T2L, RB1 and RB3 contain tRNA gene regions that are homologous to that of phage T4 but lack segB gene and several tRNA genes. In co-infections of phages T4 and T2L, segB gene is inherited with nearly 100% of efficiency. The preferred inheritance depends absolutely on the segB gene integrity and is accompanied by the loss of the T2L tRNA gene region markers. We suggest that SegB is a homing endonuclease that functions to ensure spreading of its own gene and the surrounding tRNA genes among T4-related phages.
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