ArticleNucleic acids research2008
Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 82 citations in OpenAlex.
- The eutherian-specific histone H3.4 promotes germ cell development and reproductive fitness.Nature communications · 2026Article
- Fam170a deficiency causes male infertility by impairing histone-to-protamine exchange during mouse spermiogenesis.Nucleic acids research · 2025Article
- Nucleosome assembly protein 1 like 1 (NAP1L1) promotes cardiac fibrosis by inhibiting YAP1 ubiquitination and degradation.MedComm · 2023Article
- Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1.Nature structural & molecular biology · 2023Article
- SHP-1 dephosphorylates histone H2B to facilitate its ubiquitination during transcription.The EMBO journal · 2022Article
- The Art of Packaging the Sperm Genome: Molecular and Structural Basis of the Histone-To-Protamine Exchange.Frontiers in endocrinology · 2022Review
- Structural studies of functional nucleosome complexes with transacting factors.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Review
- Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay.eLife · 2021Article
- The dark side of histones: genomic organization and role of oncohistones in cancer.Clinical epigenetics · 2021Review
- Histone variants at a glance.Journal of cell science · 2021Review
- NAP1L5 Promotes Nucleolar Hypertrophy and Is Required for Translation Activation During Cardiomyocyte Hypertrophy.Frontiers in cardiovascular medicine · 2021Article
- Article
- Replication-dependent histone isoforms: a new source of complexity in chromatin structure and function.Nucleic acids research · 2018Review
- Histone H2A variants confer specific properties to nucleosomes and impact on chromatin accessibility.Nucleic acids research · 2018Article
- The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation.Scientific reports · 2018Article
- Regulation of DNA replication-coupled histone gene expression.Oncotarget · 2017Review
- Identification of the amino acid residues responsible for stable nucleosome formation by histone H3.Y.Nucleus (Austin, Tex.) · 2017Article
- A wheat histone variant gene TaH2A.7 enhances drought tolerance and promotes stomatal closure in Arabidopsis.Plant cell reports · 2016Article
- Structure and function of human histone H3.Y nucleosome.Nucleic acids research · 2016Article
- Epigenetic regulation of the histone-to-protamine transition during spermiogenesis.Reproduction (Cambridge, England) · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five non-allelic histone H3 variants, H3.1, H3.2, H3.3, H3t and CENP-A, have been identified in mammals. H3t is robustly expressed in the testis, and thus was assigned as the testis-specific H3 variant. However, recent proteomics and tissue-specific RT-PCR experiments revealed a small amount of H3t expression in somatic cells. In the present study, we purified human H3t as a recombinant protein, and showed that H3t/H4 forms nucleosomes with H2A/H2B by the salt-dialysis method, like the conventional H3.1/H4. We found that H3t/H4 is not efficiently incorporated into the nucleosome by human Nap1 (hNap1), due to its defective H3t/H4 deposition on DNA. In contrast, human Nap2 (hNap2), a paralog of hNap1, promotes nucleosome assembly with H3t/H4. Mutational analyses revealed that the Ala111 residue, which is conserved among H3.1, H3.2 and H3.3, but not in H3t, is the essential residue for the hNap1-mediated nucleosome assembly. These results suggest that H3t may be incorporated into chromatin by a specific chaperone-mediated pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.