ArticleInvestigative ophthalmology & visual science2008
Real-time in vivo imaging of retinal cell apoptosis after laser exposure.
Article in Investigative ophthalmology & visual science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Real-time imaging of single neuronal cell apoptosis in patients with glaucoma.Brain : a journal of neurology · 2017Trial
- The roles of glycerophospholipids in the aging retina and age-related macular degeneration.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2026Review
- Alzheimer's disease pathophysiology in the Retina.Progress in retinal and eye research · 2024Review
- The basic science of optic nerve regeneration.Annals of translational medicine · 2021Review
- Laser-Activated Drug Implant for Controlled Release to the Posterior Segment of the Eye.ACS applied bio materials · 2021Article
- Imaging of Therapeutic Effects of Anti-Vascular Endothelial Growth Factor Inhibitors by Optical Coherence Tomography Angiography in a Rat Model.Translational vision science & technology · 2020Article
- Retinal safety evaluation of two-photon laser scanning in rats.Biomedical optics express · 2019Article
- Review
- [Optical coherence tomography angiography (OCT‑A) in rats].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2017Article
- Evaluating retinal ganglion cell loss and dysfunction.Experimental eye research · 2016Review
- Phosphatidylserine (PS) Is Exposed in Choroidal Neovascular Endothelium: PS-Targeting Antibodies Inhibit Choroidal Angiogenesis In Vivo and Ex Vivo.Investigative ophthalmology & visual science · 2015Article
- Advances in retinal ganglion cell imaging.Eye (London, England) · 2015Review
- Current perspective of neuroprotection and glaucoma.Clinical ophthalmology (Auckland, N.Z.) · 2015Review
- Molecular imaging of retinal disease.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2013Review
- Retrograde and Wallerian axonal degeneration occur synchronously after retinal ganglion cell axotomy.The American journal of pathology · 2012Article
- Retinal photoreceptor focal disruption secondary to accidental Nd:YAG laser exposure.International ophthalmology · 2011Article
- Imaging apoptosis in the eye.Eye (London, England) · 2011Article
- Glaucoma: genes, phenotypes, and new directions for therapy.The Journal of clinical investigation · 2010Review
- Phosphatidylserine targeting for diagnosis and treatment of human diseases.Apoptosis : an international journal on programmed cell death · 2010Review
- Monitoring apoptosis and neuronal degeneration by real-time detection of phosphatidylserine externalization using a polarity-sensitive indicator of viability and apoptosis.Nature protocols · 2010Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
purposeTo investigate whether the detection of apoptosing retinal cells (DARC) could detect cells undergoing apoptosis in a laser model of retinal damage.
methodsLaser lesions were placed, with the use of a frequency-doubled Nd:YAG laser, on the retina in 34 eyes of anesthetized Dark Agouti rats. Lesion size and laser-induced retinal elevation were analyzed using in vivo reflectance imaging. Development of retinal cell apoptosis was assessed using intravitreal fluorescence-labeled annexin 5 in vivo with DARC technology from baseline until 90 minutes after laser application. Histologic analysis of retinal flat mounts and cross-sections was performed.
resultsThe lateral and anteroposterior depth extension of the zone of laser damage was significantly larger for higher exposure settings. A strong diffuse signal, concentrated at the outer retina, was seen with DARC for low exposures (<300 ms and <300 mW). In comparison, higher exposures (>300 ms and >300 mW) resulted in detectable hyperfluorescent spots, mainly at the level of the inner retinal layers. Dose-dependent effects on spot density and positive correlation of spot density between lesion size (P < 0.0001) and retinal elevation (P < 0.0001) were demonstrated. Histology confirmed the presence of apoptosing retinal cells in the inner nuclear and the ganglion cell layers.
conclusionsThis is the first time that DARC has been used to determine apoptotic effects in the inner nuclear layer. The ability to monitor changes spatially and temporally in vivo promises to be a major advance in the real-time assessment of retinal diseases and treatment effects.
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